Anti-SLC5A7 antibody (503-580) (STJ11100952)
SPECIFICATIONS
ClonalityPolyclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
General Information
| Short Description | Rabbit polyclonal SLC5A7 (503-580) antibody for use in WB, IF, ICC and ELISA in human, mouse and rat samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications | WB/IF/ICC/ELISA |
| Host | Rabbit |
| Reactivity | Human/Mouse/Rat |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Concentration | Lot specific |
| Purification | Affinity purification |
| Dilution Range | WB:1:500-1:2000IF/ICC:1:50-1:200ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements. |
| Formulation | PBS with 0.02% Sodium Azide, 50% Glycerol, pH 7.3. |
| Storage Instruction | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
Target Information
| Gene Symbol | SLC5A7 |
| Gene ID | 60482 |
| Uniprot ID | SC5A7_HUMAN |
| Immunogen Region | 503-580 |
| Immunogen Sequence | ESGTLPPKLDVFDAVVARHS EENMDKTILVKNENIKLDEL ALVKPRQSMTLSSTFTNKEA FLDVDSSPEGSGTEDNLQ |
| Specificity | Recombinant fusion protein containing a sequence corresponding to amino acids 503-580 of human SLC5A7 (NP_068587.1). |
Additional Info
| Tissue Specificity | Expressed in putamen, spinal cord and medulla. Expressed in cholinergic neurons. |
| Post Translational Modifications | Phosphorylated. |
| Function | High-affinity Na(+)-coupled choline transmembrane symporter. Functions as an electrogenic, voltage-dependent transporter with variable charge/choline stoichiometry. Choline uptake and choline-induced current is also Cl(-)-dependent where Cl(-) is likely a regulatory ion rather than cotransported ion. Plays a critical role in acetylcholine (ACh) synthesis by taking up the substrate choline from the synaptic cleft into the presynaptic nerve terminals after neurotransmitter release. SLC5A7/CHT1-mediated choline high-affinity transport in cholinergic neurons is the rate-limiting step for production of ACh, thereby facilitating communication by subsequent action potentials. Localized predominantly in presynaptic terminal intracellular organelles, and translocated to the plasma membrane in active form in response to neuronal activity. |
| Protein Name | High Affinity Choline Transporter 1Hcht1Hemicholinium-3-Sensitive Choline TransporterChtSolute Carrier Family 5 Member 7 |
| Database Links | Reactome: R-HSA-264642Reactome: R-HSA-425366Reactome: R-HSA-5619114Reactome: R-HSA-5658471 |
| Cellular Localisation | Presynaptic Cell MembraneMulti-Pass Membrane ProteinCell ProjectionAxonEarly Endosome MembraneCytoplasmic VesicleSecretory VesicleSynaptic Vesicle MembraneLocalized At The Neuromuscular JunctionLocalization At The Plasma Membrane Is Transient Due To The Rapid Endocytosis Of Slc5a7/Cht1 Via The Clathrin-Mediated PathwayWhere It Localizes To Early EndosomesAlso Localized To Synaptic Vesicles Where It Is Likely Mobilized To The Cell Surface By Exocytosis |
| Alternative Antibody Names | Anti-High Affinity Choline Transporter 1 antibodyAnti-Hcht1 antibodyAnti-Hemicholinium-3-Sensitive Choline Transporter antibodyAnti-Cht antibodyAnti-Solute Carrier Family 5 Member 7 antibodyAnti-SLC5A7 antibodyAnti-CHT1 antibody |
Information sourced from Uniprot.org