• Western blot analysis of lysates from Mouse brain using SLC5A3 Rabbit polyclonal antibody (STJ11100333) at 1:2000 dilution. Secondary antibody: (STJS000856) at 1:10000 dilution. Lysates/proteins: 25  Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 5s.

Anti-SLC5A3 antibody (535-695) (STJ11100333)

SKU:
STJ11100333

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Host: Rabbit
Applications: WB
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-SLC5A3 (535-695) is suitable for use in Western Blot research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.01% Thimerosal, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: SLC5A3Uniprot ID=SC5A3_HUMAN"
Immunogen Region: 535-695
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 535-695 of human SLC5A3 (NP_008864.4).
Immunogen Sequence: PPTKEQIRTTTFWSKKNLVV KENCSPKEEPYKMQEKSILR CSENNETINHIIPNGKSEDS IKGLQPEDVNLLVTCREEGN PVASLGHSEAETPVDAYSNG QAALMGEKERKKETDDGGRY WKFIDWFCGFKSKSLSKRSL RDLMEEEAVCLQMLEETRQV K
Function Electrogenic Na(+)-coupled sugar symporter that actively transports myo-inositol and its stereoisomer scyllo-inositol across the plasma membrane, with a Na(+) to sugar coupling ratio of 2:1. Maintains myo-inositol concentration gradient that defines cell volume and fluid balance during osmotic stress, in particular in the fetoplacental unit and central nervous system. Forms coregulatory complexes with voltage-gated K(+) ion channels, allosterically altering ion selectivity, voltage dependence and gating kinetics of the channel. In turn, K(+) efflux through the channel forms a local electrical gradient that modulates electrogenic Na(+)-coupled myo-inositol influx through the transporter. Associates with KCNQ1-KCNE2 channel in the apical membrane of choroid plexus epithelium and regulates the myo-inositol gradient between blood and cerebrospinal fluid with an impact on neuron excitability. Associates with KCNQ2-KCNQ3 channel altering ion selectivity, increasing Na(+) and Cs(+) permeation relative to K(+) permeation. Provides myo-inositol precursor for biosynthesis of phosphoinositides such as PI(4,5)P2, thus indirectly affecting the activity of phosphoinositide-dependent ion channels and Ca(2+) signaling upon osmotic stress.
Protein Name Sodium/Myo-Inositol Cotransporter
Na(+/Myo-Inositol Cotransporter
Sodium/Myo-Inositol Transporter 1
Smit1
Solute Carrier Family 5 Member 3
Cellular Localisation Apical Cell Membrane
Multi-Pass Membrane Protein
Basolateral Cell Membrane
Colocalizes With Kcnq1 At The Apical Membrane Of Choroid Plexus Epithelium
Alternative Antibody Names Anti-Sodium/Myo-Inositol Cotransporter antibody
Anti-Na(+/Myo-Inositol Cotransporter antibody
Anti-Sodium/Myo-Inositol Transporter 1 antibody
Anti-Smit1 antibody
Anti-Solute Carrier Family 5 Member 3 antibody
Anti-SLC5A3 antibody

Information sourced from Uniprot.org

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