Anti-SLC5A3 antibody (535-695) (STJ11100333)
SPECIFICATIONS
ClonalityPolyclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
General Information
| Short Description | Rabbit polyclonal SLC5A3 (535-695) antibody for use in WB and ELISA in human, mouse and rat samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications | WB/ELISA |
| Host | Rabbit |
| Reactivity | Human/Mouse/Rat |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Concentration | Lot specific |
| Purification | Affinity purification |
| Dilution Range | WB:1:500-1:2000ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements. |
| Formulation | PBS with 0.09% Sodium Azide, 50% Glycerol, pH7.3. |
| Storage Instruction | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
Target Information
| Gene Symbol | SLC5A3 |
| Gene ID | 6526 |
| Uniprot ID | SC5A3_HUMAN |
| Immunogen Region | 535-695 |
| Immunogen Sequence | PPTKEQIRTTTFWSKKNLVV KENCSPKEEPYKMQEKSILR CSENNETINHIIPNGKSEDS IKGLQPEDVNLLVTCREEGN PVASLGHSEAETPVDAYSNG QAALMGEKERKKETDDGGRY WKFIDWFCGFKSKSLSKRSL RDLMEEEAVCLQMLEETRQV K |
| Specificity | Recombinant fusion protein containing a sequence corresponding to amino acids 535-695 of human SLC5A3 (NP_008864.4). |
Additional Info
| Function | Electrogenic Na(+)-coupled sugar symporter that actively transports myo-inositol and its stereoisomer scyllo-inositol across the plasma membrane, with a Na(+) to sugar coupling ratio of 2:1. Maintains myo-inositol concentration gradient that defines cell volume and fluid balance during osmotic stress, in particular in the fetoplacental unit and central nervous system. Forms coregulatory complexes with voltage-gated K(+) ion channels, allosterically altering ion selectivity, voltage dependence and gating kinetics of the channel. In turn, K(+) efflux through the channel forms a local electrical gradient that modulates electrogenic Na(+)-coupled myo-inositol influx through the transporter. Associates with KCNQ1-KCNE2 channel in the apical membrane of choroid plexus epithelium and regulates the myo-inositol gradient between blood and cerebrospinal fluid with an impact on neuron excitability. Associates with KCNQ2-KCNQ3 channel altering ion selectivity, increasing Na(+) and Cs(+) permeation relative to K(+) permeation. Provides myo-inositol precursor for biosynthesis of phosphoinositides such as PI(4,5)P2, thus indirectly affecting the activity of phosphoinositide-dependent ion channels and Ca(2+) signaling upon osmotic stress. |
| Protein Name | Sodium/Myo-Inositol CotransporterNa(+/Myo-Inositol CotransporterSodium/Myo-Inositol Transporter 1Smit1Solute Carrier Family 5 Member 3 |
| Database Links | Reactome: R-HSA-429593 |
| Cellular Localisation | Apical Cell MembraneMulti-Pass Membrane ProteinBasolateral Cell MembraneColocalizes With Kcnq1 At The Apical Membrane Of Choroid Plexus Epithelium |
| Alternative Antibody Names | Anti-Sodium/Myo-Inositol Cotransporter antibodyAnti-Na(+/Myo-Inositol Cotransporter antibodyAnti-Sodium/Myo-Inositol Transporter 1 antibodyAnti-Smit1 antibodyAnti-Solute Carrier Family 5 Member 3 antibodyAnti-SLC5A3 antibody |
Information sourced from Uniprot.org