• STJ71842 Immunofluorescence analysis of paraformaldehyde fixed HepG2 cells, permeabilized with 0. 15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml) , showing membrane staining. The nuclear stain is DAPI (blue). NA NA NA Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml).
  • STJ71842 (0. 1µg/ml) staining of Human Liver lysate (35µg protein in RIPA buffer). Detected by chemiluminescence.

Anti-SLC47A1 antibody (Internal) (STJ71842)

SKU:
STJ71842-100

£303.50
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Host: Goat
Applications: Pep-ELISA/IF/WB
Reactivity: Human
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Goat polyclonal antibody anti-SLC47A1 (Internal) is suitable for use in ELISA, Immunofluorescence and Western Blot research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. NA
Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Concentration: 0.5 mg/mL
Dilution Range: WB-0.1-0.3µg/ml
IF-Strong expression of the protein seen in the plasma membrane of HepG2 cells. Recommened concentration: 10µg/ml
ELISA-antibody detection limit dilution 1:64000.
Storage Instruction: Store at-20°C on receipt and minimise freeze-thaw cycles.
Gene Symbol: SLC47A1
Gene ID: 55244
Uniprot ID: S47A1_HUMAN
Immunogen Region: Internal
Accession Number: NP_060712.2
Immunogen Sequence: PEHPQDGAKLSRKQ
Function Multidrug efflux pump that functions as a H(+)/organic cation antiporter. Plays a physiological role in the excretion of cationic compounds including endogenous metabolites, drugs, toxins through the kidney and liver, into urine and bile respectively. Mediates the efflux of endogenous compounds such as creatinine, vitamin B1/thiamine, agmatine and estrone-3-sulfate. May also contribute to regulate the transport of cationic compounds in testis across the blood-testis-barrier (Probable).
Protein Name Multidrug And Toxin Extrusion Protein 1
Mate-1
Hmate-1
Solute Carrier Family 47 Member 1
Database Links Reactome: R-HSA-425366
Cellular Localisation Cell Membrane
Multi-Pass Membrane Protein
Apical Cell Membrane
Localizes To The Plasma Membrane
At The Brush Border Membranes Of The Proximal Tubules (Kidney) And At The Bile Caniculi (Liver)
Alternative Antibody Names Anti-Multidrug And Toxin Extrusion Protein 1 antibody
Anti-Mate-1 antibody
Anti-Hmate-1 antibody
Anti-Solute Carrier Family 47 Member 1 antibody
Anti-SLC47A1 antibody
Anti-MATE1 antibody

Information sourced from Uniprot.org

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