Anti-SLC40A1 antibody (245-259) (STJ73127)
SPECIFICATIONS
ClonalityPolyclonal
HostGoat
ConjugationUnconjugated
IsotypeIgG
General Information
| Short Description | Goat polyclonal antibody anti-SLC40A1 (245-259) is suitable for use in ELISA, Immunofluorescence and Flow Cytometry research applications. |
| Applications | Pep-ELISA/IF/FC |
| Host | Goat |
| Reactivity | Human |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Concentration | 0.5 mg/mL |
| Purification | Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. |
| Dilution Range | Peptide ELISA: antibody detection limit dilution 1:128000.WB: Preliminary testing showed a band at approx 60kDa in Human Breast and Colonr lysate after 1-2µg/ml antibody staining (calculated MW of 62.5kDa according to NP_055400.1). Primary incub |
| Formulation | 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. NA |
| Storage Instruction | Store at-20°C on receipt and minimise freeze-thaw cycles. |
Target Information
| Gene Symbol | SLC40A1 |
| Gene ID | 30061 |
| Uniprot ID | S40A1_HUMAN |
| Accession Number | NP_055400.1 |
| Immunogen Region | 245-259 |
| Immunogen Sequence | ELKQLNLHKDTEPKP |
Additional Info
| Post Translational Modifications | Polyubiquitinated by RNF217.leading to proteasomal degradation. Under conditions of high systemic iron levels, both the hormone peptide hepcidin/HAMP and holo(iron bound)-transferrin/TF induce the ubiquitination, internalization and proteasomal degradation of SLC40A1 to control iron release from cells. |
| Function | Transports Fe(2+) from the inside of a cell to the outside of the cell, playing a key role for maintaining systemic iron homeostasis. Transports iron from intestinal, splenic, hepatic cells, macrophages and erythrocytes into the blood to provide iron to other tissues. Controls therefore dietary iron uptake, iron recycling by macrophages and erythrocytes, and release of iron stores in hepatocytes. When iron is in excess in serum, circulating HAMP/hepcidin levels increase resulting in a degradation of SLC40A1, thus limiting the iron efflux to plasma. |
| Protein Name | FerroportinFerroportin-1Iron-Regulated Transporter 1Solute Carrier Family 40 Member 1 |
| Database Links | Reactome: R-HSA-425410Reactome: R-HSA-5619049Reactome: R-HSA-5619060Reactome: R-HSA-5655799Reactome: R-HSA-917937 |
| Cellular Localisation | Cell MembraneMulti-Pass Membrane ProteinBasolateral Cell MembraneLocalized To The Basolateral Membrane Of Hepatocytoma Wif-B Cells |
| Alternative Antibody Names | Anti-Ferroportin antibodyAnti-Ferroportin-1 antibodyAnti-Iron-Regulated Transporter 1 antibodyAnti-Solute Carrier Family 40 Member 1 antibodyAnti-SLC40A1 antibodyAnti-FPN antibodyAnti-FPN1 antibodyAnti-IREG1 antibodyAnti-SLC11A3 antibodyAnti-MSTP079 antibody |
Information sourced from Uniprot.org