Anti-SLC39A4 antibody (Internal) (STJ71746)
SPECIFICATIONS
ClonalityPolyclonal
HostGoat
ConjugationUnconjugated
IsotypeIgG
General Information
| Short Description | Goat polyclonal antibody anti-SLC39A4 (Internal) is suitable for use in ELISA research applications. |
| Applications | Pep-ELISA |
| Host | Goat |
| Reactivity | Human |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Concentration | 0.5 mg/mL |
| Purification | Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. |
| Dilution Range | Peptide ELISA: antibody detection limit dilution 1:4000.WB: Preliminary experiments in Human Colon and Ileum lysates gave no specific signal but low background (at antibody concentration up to 1µg/ml). We would appreciate any feedback from peopl |
| Formulation | 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. NA |
| Storage Instruction | Store at-20°C on receipt and minimise freeze-thaw cycles. |
Target Information
| Gene Symbol | SLC39A4 |
| Gene ID | 55630 |
| Uniprot ID | S39A4_HUMAN |
| Accession Number | NP_060237.2; NP_570901.2 |
| Immunogen Region | Internal |
| Immunogen Sequence | RQPKPPHEGSRAD |
| Specificity | This antibody is expected to recognize both reported isoforms (NP_060237.2; NP_570901.2) |
Additional Info
| Post Translational Modifications | The extracellular N-terminal ectodomain is cleaved when cells are Zn(2+) deficient, N-terminally cleaved SLC39A4 is internalized at a faster rate. Under excess Zn(2+) conditions, SLC39A4 on the cell surface is rapidly endocytosed, ubiquitinated and degraded. Glycosylated. |
| Function | Selective transporter that mediates the uptake of Zn(2+). Plays an essential role for dietary zinc uptake from small intestine. The Zn(2+) uniporter activity is regulated by zinc availability. Exhibits also polyspecific binding and transport of Cu(2+), Cd(2+) and possibly Ni(2+) but at higher concentrations. |
| Protein Name | Zinc Transporter Zip4Solute Carrier Family 39 Member 4Zrt- And Irt-Like Protein 4Zip-4 |
| Database Links | Reactome: R-HSA-442380Reactome: R-HSA-5619088 |
| Cellular Localisation | Cell MembraneMulti-Pass Membrane ProteinRecycling Endosome MembraneApical Cell MembraneColocalized With Tfrc In The Recycling EndosomesCycles Between Endosomal Compartments And The Plasma Membrane In Response To Zn(2+) AvailabilityZn(2+) Deficiency Promotes Accumulation Of Slc39a4 On The Surface MembraneWhereas High Extracellular Zn(2+) Levels Induce Internalization Of Slc39a4But Also Trigger Drastic Removal Of Cellular Slc39a4 Via Proteasomal And Lysosomal Degradation Pathways |
| Alternative Antibody Names | Anti-Zinc Transporter Zip4 antibodyAnti-Solute Carrier Family 39 Member 4 antibodyAnti-Zrt- And Irt-Like Protein 4 antibodyAnti-Zip-4 antibodyAnti-SLC39A4 antibodyAnti-ZIP4 antibody |
Information sourced from Uniprot.org