• Immunohistochemistry analysis of SLC39A4 in paraffin-embedded mouse lung using SLC39A4 Rabbit polyclonal antibody (STJ25583) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of SLC39A4 in paraffin-embedded rat liver using SLC39A4 Rabbit polyclonal antibody (STJ25583) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of SLC39A4 in paraffin-embedded human colon carcinoma using SLC39A4 Rabbit polyclonal antibody (STJ25583) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Western blot analysis of lysates from wild type (WT) and 293T cells transfected with SLC39A4 using SLC39A4 Rabbit polyclonal antibody (STJ25583) at 1:2000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.

Anti-SLC39A4 antibody (23-327) (STJ25583)

SKU:
STJ25583

£169.50 - £449.50
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Host: Rabbit
Applications: WB/IHC
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-SLC39A4 (23-327) is suitable for use in Western Blot and Immunohistochemistry research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:1000-1:5000
IHC-P 1:50-1:100
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: SLC39A4
Gene ID: 55630
Uniprot ID: S39A4_HUMAN
Immunogen Region: 23-327
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 23-327 of human SLC39A4 (NP_570901.2).
Immunogen Sequence: SPPAGLLSLLTSGQGALDQE ALGGLLNTLADRVHCANGPC GKCLSVEDALGLGEPEGSGL PPGPVLEARYVARLSAAAVL YLSNPEGTCEDARAGLWASH ADHLLALLESPKALTPGLSW LLQRMQARAAGQTPKTACVD IPQLLEEAVGAGAPGSAGGV LAALLDHVRSGSCFHALPSP QYFVDFVFQQHSSEVPMTLA ELSALMQRLGVGREAHSDHS HRHRGASSRDPVPLISSSN
Tissue Specificity Highly expressed in kidney, small intestine, stomach, colon, jejunum and duodenum.
Post Translational Modifications The extracellular N-terminal ectodomain is cleaved when cells are Zn(2+) deficient, N-terminally cleaved SLC39A4 is internalized at a faster rate. Under excess Zn(2+) conditions, SLC39A4 on the cell surface is rapidly endocytosed, ubiquitinated and degraded. Glycosylated.
Function Selective transporter that mediates the uptake of Zn(2+). Plays an essential role for dietary zinc uptake from small intestine. The Zn(2+) uniporter activity is regulated by zinc availability. Exhibits also polyspecific binding and transport of Cu(2+), Cd(2+) and possibly Ni(2+) but at higher concentrations.
Protein Name Zinc Transporter Zip4
Solute Carrier Family 39 Member 4
Zrt- And Irt-Like Protein 4
Zip-4
Database Links Reactome: R-HSA-442380
Reactome: R-HSA-5619088
Cellular Localisation Cell Membrane
Multi-Pass Membrane Protein
Recycling Endosome Membrane
Apical Cell Membrane
Colocalized With Tfrc In The Recycling Endosomes
Cycles Between Endosomal Compartments And The Plasma Membrane In Response To Zn(2+) Availability
Zn(2+) Deficiency Promotes Accumulation Of Slc39a4 On The Surface Membrane
Whereas High Extracellular Zn(2+) Levels Induce Internalization Of Slc39a4
But Also Trigger Drastic Removal Of Cellular Slc39a4 Via Proteasomal And Lysosomal Degradation Pathways
Alternative Antibody Names Anti-Zinc Transporter Zip4 antibody
Anti-Solute Carrier Family 39 Member 4 antibody
Anti-Zrt- And Irt-Like Protein 4 antibody
Anti-Zip-4 antibody
Anti-SLC39A4 antibody
Anti-ZIP4 antibody

Information sourced from Uniprot.org

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