| Host: |
Rabbit |
| Applications: |
WB/IHC/IF |
| Reactivity: |
Human/Mouse/Rat |
| Note: |
STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description: |
Rabbit polyclonal antibody anti-ZIP14 (138-152) is suitable for use in Western Blot, Immunohistochemistry and Immunofluorescence research applications. |
| Clonality: |
Polyclonal |
| Conjugation: |
Unconjugated |
| Isotype: |
IgG |
| Formulation: |
PBS with 0.05% Proclin300, 50% Glycerol, pH7.3. |
| Purification: |
Affinity purification |
| Dilution Range: |
WB 1:500-1:1000IHC-P 1:50-1:200IF/ICC 1:50-1:200 |
| Storage Instruction: |
Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: |
SLC39A14 |
| Gene ID: |
23516 |
| Uniprot ID: |
S39AE_HUMAN |
| Immunogen Region: |
138-152 |
| Immunogen: |
Recombinant fusion protein containing a sequence corresponding to amino acids 138-152 of human ZIP14 (NP_001121903.1). |
| Immunogen Sequence: |
EENEQTEEGRPSAVE |
| Tissue Specificity | Ubiquitously expressed, with higher expression in liver, pancreas, fetal liver, thyroid gland, left and right ventricle, right atrium and fetal heart. Weakly expressed in spleen, thymus, and peripheral blood leukocytes. Expressed in liver and in brain by large neurons in the globus pallidus, the insular cortex and the dentate nucleus and to a lower extent in the putamen and the caudate nucleus (at protein level). Expressed in osteoblasts and giant osteoclast-like cells, but not in osteocytes found osteoblastoma and giant cell tumors (at protein level). Expressed by microvascular capillary endothelial cells that constitute the blood-brain barrier (at protein level). Expressed by macrophages. Isoform 2: Widely expressed but not detected in brain, heart, skeletal muscle, placenta and fetal skin. |
| Post Translational Modifications | Ubiquitinated. Ubiquitination occurs upon iron depletion. The ubiquitinated form undergoes proteasomal degradation. N-glycosylated. N-glycosylation at Asn-102 is required for iron-regulated extraction of the transporter from membranes and subsequent proteasomal degradation. |
| Function | Electroneutral transporter of the plasma membrane mediating the cellular uptake of the divalent metal cations zinc, manganese and iron that are important for tissue homeostasis, metabolism, development and immunity. Functions as an energy-dependent symporter, transporting through the membranes an electroneutral complex composed of a divalent metal cation and two bicarbonate anions. Beside these endogenous cellular substrates, can also import cadmium a non-essential metal which is cytotoxic and carcinogenic. Controls the cellular uptake by the intestinal epithelium of systemic zinc, which is in turn required to maintain tight junctions and the intestinal permeability. Modifies the activity of zinc-dependent phosphodiesterases, thereby indirectly regulating G protein-coupled receptor signaling pathways important for gluconeogenesis and chondrocyte differentiation. Regulates insulin receptor signaling, glucose uptake, glycogen synthesis and gluconeogenesis in hepatocytes through the zinc-dependent intracellular catabolism of insulin. Through zinc cellular uptake also plays a role in the adaptation of cells to endoplasmic reticulum stress. Major manganese transporter of the basolateral membrane of intestinal epithelial cells, it plays a central role in manganese systemic homeostasis through intestinal manganese uptake. Also involved in manganese extracellular uptake by cells of the blood-brain barrier. May also play a role in manganese and zinc homeostasis participating in their elimination from the blood through the hepatobiliary excretion. Also functions in the extracellular uptake of free iron. May also function intracellularly and mediate the transport from endosomes to cytosol of iron endocytosed by transferrin. Plays a role in innate immunity by regulating the expression of cytokines by activated macrophages. |
| Protein Name | Metal Cation Symporter Zip14Liv-1 Subfamily Of Zip Zinc Transporter 4Lzt-Hs4Solute Carrier Family 39 Member 14Zrt- And Irt-Like Protein 14Zip-14 |
| Database Links | Reactome: R-HSA-442380 |
| Cellular Localisation | Cell MembraneMulti-Pass Membrane ProteinApical Cell MembraneBasolateral Cell MembraneEarly Endosome MembraneLate Endosome MembraneLysosome MembraneLocalized And Functional At Both Apical And Basolateral Membranes Of Microvascular Capillary Endothelial Cells That Constitute The Blood-Brain BarrierLocalized At The Basolateral Membrane Of EnterocytesEnriched At The Plasma Membrane Upon Glucose Uptake |
| Alternative Antibody Names | Anti-Metal Cation Symporter Zip14 antibodyAnti-Liv-1 Subfamily Of Zip Zinc Transporter 4 antibodyAnti-Lzt-Hs4 antibodyAnti-Solute Carrier Family 39 Member 14 antibodyAnti-Zrt- And Irt-Like Protein 14 antibodyAnti-Zip-14 antibodyAnti-SLC39A14 antibodyAnti-KIAA0062 antibodyAnti-ZIP14 antibody |
Information sourced from Uniprot.org
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
