• STJ73720 Immunofluorescence analysis of paraformaldehyde fixed NIH3T3 cells, permeabilized with 0. 15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml) , showing membrane and cytoplasmic staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml).

Anti-SLC2A4 antibody (Internal) (STJ73720)

SKU:
STJ73720-100

£303.50
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Host: Goat
Applications: Pep-ELISA/IF
Reactivity: Human/Mouse/Rat/Pig/Rabbit/Dog/Cow
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Goat polyclonal antibody anti-SLC2A4 (Internal) is suitable for use in ELISA and Immunofluorescence research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. NA
Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Concentration: 0.5 mg/mL
Dilution Range: IF-Immunofluorescence: Strong expression of the protein seen in the membrane and cytoplasm of NIH3T3 cells. 10µg/ml
ELISA-antibody detection limit dilution 1:16000.
Storage Instruction: Store at-20°C on receipt and minimise freeze-thaw cycles.
Gene Symbol: SLC2A4
Gene ID: 6517
Uniprot ID: GLUT4_HUMAN
Immunogen Region: Internal
Accession Number: NP_001033.1
Immunogen Sequence: NLEGPARKSLKRLT
Post Translational Modifications Sumoylated. Palmitoylated. Palmitoylation by ZDHHC7 controls the insulin-dependent translocation of GLUT4 to the plasma membrane.
Function Insulin-regulated facilitative glucose transporter, which plays a key role in removal of glucose from circulation. Response to insulin is regulated by its intracellular localization: in the absence of insulin, it is efficiently retained intracellularly within storage compartments in muscle and fat cells. Upon insulin stimulation, translocates from these compartments to the cell surface where it transports glucose from the extracellular milieu into the cell.
Protein Name Solute Carrier Family 2 - Facilitated Glucose Transporter Member 4
Glucose Transporter Type 4 - Insulin-Responsive
Glut-4
Database Links Reactome: R-HSA-1445148
Reactome: R-HSA-189200
Reactome: R-HSA-381340
Cellular Localisation Cell Membrane
Multi-Pass Membrane Protein
Endomembrane System
Cytoplasm
Perinuclear Region
Localizes Primarily To The Perinuclear Region
Undergoing Continued Recycling To The Plasma Membrane Where It Is Rapidly Reinternalized
The Dileucine Internalization Motif Is Critical For Intracellular Sequestration
Insulin Stimulation Induces Translocation To The Cell Membrane
Alternative Antibody Names Anti-Solute Carrier Family 2 - Facilitated Glucose Transporter Member 4 antibody
Anti-Glucose Transporter Type 4 - Insulin-Responsive antibody
Anti-Glut-4 antibody
Anti-SLC2A4 antibody
Anti-GLUT4 antibody

Information sourced from Uniprot.org

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