• Confocal imaging of paraffin-embedded Human prostate cancer tissue using GLUT1/SLC2A1 Rabbit monoclonal antibody (STJ11101397, dilution 1:200) followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) ( dilution 1:500) (Red). DAPI was used for nuclear staining (Blue). Objective: 40x. Perform high pressure antigen retrieval with 0. 01 M citrate buffer (pH 6. 0) prior to IF staining.
  • Confocal imaging of paraffin-embedded Mouse liver tissue using GLUT1/SLC2A1 Rabbit monoclonal antibody (STJ11101397, dilution 1:200) followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) ( dilution 1:500) (Red). DAPI was used for nuclear staining (Blue). Objective: 40x. Perform high pressure antigen retrieval with 0. 01 M citrate buffer (pH 6. 0) prior to IF staining.
  • Immunohistochemistry analysis of paraffin-embedded Human placenta tissue using GLUT1/SLC2A1 Rabbit monoclonal antibody (STJ11101397) at a dilution of 1:800 (40x lens). High pressure antigen retrieval performed with 0. 01M Tris-EDTA Buffer (pH 9. 0) prior to immunohistochemistry staining.
  • Immunohistochemistry analysis of paraffin-embedded Human pancreas tissue using GLUT1/SLC2A1 Rabbit monoclonal antibody (STJ11101397) at a dilution of 1:800 (40x lens). High pressure antigen retrieval performed with 0. 01M Tris-EDTA Buffer (pH 9. 0) prior to immunohistochemistry staining.
  • Immunohistochemistry analysis of paraffin-embedded Human liver tissue using GLUT1/SLC2A1 Rabbit monoclonal antibody (STJ11101397) at a dilution of 1:800 (40x lens). High pressure antigen retrieval performed with 0. 01M Tris-EDTA Buffer (pH 9. 0) prior to immunohistochemistry staining.
  • Immunohistochemistry analysis of paraffin-embedded Human lung squamous carcinoma tissue using GLUT1/SLC2A1 Rabbit monoclonal antibody (STJ11101397) at a dilution of 1:800 (40x lens). High pressure antigen retrieval performed with 0. 01M Tris-EDTA Buffer (pH 9. 0) prior to immunohistochemistry staining.
  • Western blot analysis of various lysates using GLUT1/SLC2A1 Rabbit monoclonal antibody (STJ11101397) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.

Anti-SLC2A1 antibody (393-492) [S7MR] (STJ11101397)

SKU:
STJ11101397

£212.50 - £501.50
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Host: Rabbit
Applications: WB/IP
Reactivity: Human/Mouse
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit monoclonal antibody anti-GLUT1 (393-492) is suitable for use in Western Blot and Immunoprecipitation research applications.
Clonality: Monoclonal
Clone ID: S7MR
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 0.05% BSA, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
IP 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: SLC2A1
Gene ID: 6513
Uniprot ID: GTR1_HUMAN
Immunogen Region: 393-492
Immunogen: A synthetic peptide corresponding to a sequence within amino acids 393-492 of human GLUT1/SLC2A1 (P11166).
Immunogen Sequence: ELFSQGPRPAAIAVAGFSNW TSNFIVGMCFQYVEQLCGPY VFIIFTVLLVLFFIFTYFKV PETKGRTFDEIASGFRQGGA SQSDKTPEELFHPLGADSQV
Tissue Specificity Detected in erythrocytes (at protein level). Expressed at variable levels in many human tissues.
Post Translational Modifications Phosphorylation at Ser-226 by PKC promotes glucose uptake by increasing cell membrane localization.
Function Facilitative glucose transporter, which is responsible for constitutive or basal glucose uptake. Has a very broad substrate specificity.can transport a wide range of aldoses including both pentoses and hexoses. Most important energy carrier of the brain: present at the blood-brain barrier and assures the energy-independent, facilitative transport of glucose into the brain. In association with BSG and NXNL1, promotes retinal cone survival by increasing glucose uptake into photoreceptors. Required for mesendoderm differentiation.
Protein Name Solute Carrier Family 2 - Facilitated Glucose Transporter Member 1
Glucose Transporter Type 1 - Erythrocyte/Brain
Glut-1
Hepg2 Glucose Transporter
Database Links Reactome: R-HSA-189200
Reactome: R-HSA-196836
Reactome: R-HSA-422356
Reactome: R-HSA-5619043
Reactome: R-HSA-5653890
Cellular Localisation Cell Membrane
Multi-Pass Membrane Protein
Melanosome
Photoreceptor Inner Segment
Localizes Primarily At The Cell Surface
Identified By Mass Spectrometry In Melanosome Fractions From Stage I To Stage Iv
Alternative Antibody Names Anti-Solute Carrier Family 2 - Facilitated Glucose Transporter Member 1 antibody
Anti-Glucose Transporter Type 1 - Erythrocyte/Brain antibody
Anti-Glut-1 antibody
Anti-Hepg2 Glucose Transporter antibody
Anti-SLC2A1 antibody
Anti-GLUT1 antibody

Information sourced from Uniprot.org

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