• STJ72194 (10µg/ml) staining of Human renal cortical brush-border membrane lysate (90µg protein denatured at 37C for 30min) with (B) and without (A) blocking with the immunizing peptide. Primary incubation was overnight at 4C. Detected by NBT/BCIP. Data kindly provided by Dean Karaica, B. Sc. , and Ivan Sabolic, M. D. , Ph. D. , Institute for Medical Research and Occupational Health, Zagreb, Croatia.
  • STJ72194 (0. 1mg/ml) overnight staining of paraffin embedded Human Kidney. Microwaved antigen retrieval with citrate buffer pH 6, CY3-staining. Data kindly provided by Dean Karaica, B. Sc. , and Ivan Sabolic, M. D. , Ph. D. , Institute for Medical Research and Occupational Health, Zagreb, Croatia.

Anti-SLC26A6 antibody (Internal) (STJ72194)

SKU:
STJ72194-100

£303.50
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Host: Goat
Applications: Pep-ELISA/WB/IHC
Reactivity: Human
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Goat polyclonal antibody anti-SLC26A6 (Internal) is suitable for use in ELISA, Western Blot and Immunohistochemistry research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. NA
Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Concentration: 0.5 mg/mL
Dilution Range: WB-10µg/ml
IHC-Recommended concentration, 0.1-0.3mg/ml
ELISA-antibody detection limit dilution 1:8000.
Storage Instruction: Store at-20°C on receipt and minimise freeze-thaw cycles.
Gene Symbol: SLC26A6
Gene ID: 65010
Uniprot ID: S26A6_HUMAN
Immunogen Region: Internal
Accession Number: NP_075062.2; NP_599025.2; NP_602298.2; NP_001035544.1
Specificity: This antibody is expected to recognize isoform 1, 2, 3 and 4 (NP_075062.2; NP_599025.2; NP_602298.2; NP_001035544.1). Please note that this antibody was shown unfit for Mouse tissues both in IHC and WB.
Immunogen Sequence: SAPRTHQWRT
Post Translational Modifications Isoform 4: Phosphorylated on serine residues by PKC.the phosphorylation disrupts interaction with carbonic anhydrase CA2 and reduces bicarbonate transport activity in a phorbol myristate acetate (PMA)-induced manner. Glycosylation at Asn-167 and Asn-172 positively regulates its chloride oxalate exchanger activity.
Function Apical membrane anion-exchanger with wide epithelial distribution that plays a role as a component of the pH buffering system for maintaining acid-base homeostasis. Acts as a versatile DIDS-sensitive inorganic and organic anion transporter that mediates the uptake of monovalent anions like chloride, bicarbonate, formate and hydroxyl ion and divalent anions like sulfate and oxalate. Functions in multiple exchange modes involving pairs of these anions, which include chloride-bicarbonate, chloride-oxalate, oxalate-formate, oxalate-sulfate and chloride-formate exchange. Apical membrane chloride-bicarbonate exchanger that mediates luminal chloride absorption and bicarbonate secretion by the small intestinal brush border membrane and contributes to intracellular pH regulation in the duodenal upper villous epithelium during proton-coupled peptide absorption, possibly by providing a bicarbonate import pathway. Mediates also intestinal chloride absorption and oxalate secretion, thereby preventing hyperoxaluria and calcium oxalate urolithiasis. Transepithelial oxalate secretion, chloride-formate, chloride-oxalate and chloride-bicarbonate transport activities in the duodenum are inhibited by PKC activation in a calcium-independent manner. The apical membrane chloride-bicarbonate exchanger provides also a major route for fluid and bicarbonate secretion into the proximal tubules of the kidney as well as into the proximal part of the interlobular pancreatic ductal tree, where it mediates electrogenic chloride-bicarbonate exchange with a chloride-bicarbonate stoichiometry of 1:2, and hence will dilute and alkalinize protein-rich acinar secretion. Mediates also the transcellular sulfate absorption and oxalate secretion across the apical membrane in the duodenum and the formate ion efflux at the apical brush border of cells in the proximal tubules of kidney. Plays a role in sperm capacitation by increasing intracellular pH. Isoform 4: Apical membrane chloride-bicarbonate exchanger. Its association with carbonic anhydrase CA2 forms a bicarbonate transport metabolon.hence maximizes the local concentration of bicarbonate at the transporter site.
Protein Name Solute Carrier Family 26 Member 6
Anion Exchange Transporter
Pendrin-Like Protein 1
Pendrin-L1
Database Links Reactome: R-HSA-427601
Cellular Localisation Cell Membrane
Multi-Pass Membrane Protein
Apical Cell Membrane
Cytoplasmic Vesicle Membrane
Microsome
Localized In Sperm Membranes
Colocalizes With Cftr At The Midpiece Of Sperm Tail
Localizes To The Apical Membrane Brush Border Of Epithelial Cells In The Proximal Tubules Of Kidney
Of Enterocytes Of The Small Intestine And Of Gastric Parietal Cells In The Stomach
Isoform 4: Cell Membrane
Basolateral Cell Membrane
Localizes To The Apical And Basolateral Surfaces Of Tubular Wall Cells In Kidney And In The Brush Border Of Pancreatic Duct Cells
Colocalized With Ca2 At The Surface Of The Cell Membrane In Order To Form A Bicarbonate Transport Metabolon
Colocalization Is Reduced In Phorbol Myristate Acetate (Pma)-Induced Cells
May Be Translocated From The Cytosolic Surface Of The Cell Membrane To The Intracellular Space By Pkc In Phorbol Myristate Acetate (Pma)-Induced Cells
Isoform 5: Cell Membrane
Isoform 6: Cell Membrane
Alternative Antibody Names Anti-Solute Carrier Family 26 Member 6 antibody
Anti-Anion Exchange Transporter antibody
Anti-Pendrin-Like Protein 1 antibody
Anti-Pendrin-L1 antibody
Anti-SLC26A6 antibody

Information sourced from Uniprot.org

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