• Western blot analysis of lysates from HepG2 and 293 cells, using SLC25A6 Antibody. The lane on the right is blocked with the synthesized peptide.
  • Western blot analysis of the lysates from K562 cells using SLC25A6 antibody.
  • Western blot analysis of various cells using ANT3 Polyclonal Antibody diluted at 1:2000

Anti-SLC25A6 antibody (121-170 aa) (STJ91606)

SKU:
STJ91606

Current Stock:
Host: Rabbit
Applications: WB/IHC/IF/ELISA
Reactivity: Human/Rat/Mouse
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-ADP/ATP translocase 3 (121-170 aa) is suitable for use in Western Blot, Immunohistochemistry, Immunofluorescence and ELISA research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: Liquid in PBS containing 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide.
Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Concentration: 1 mg/mL
Dilution Range: WB 1:500-1:2000
IHC 1:100-1:300
ELISA 1:40000
IF 1:50-200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: SLC25A6
Gene ID: 293
Uniprot ID: ADT3_HUMAN
Immunogen Region: 121-170 aa
Specificity: ANT3 Polyclonal Antibody detects endogenous levels of ANT3 protein.
Immunogen: The antiserum was produced against synthesized peptide derived from the human SLC25A6 at the amino acid range 121-170
Post Translational Modifications Trimethylated by ANTKMT at Lys-52.
Function ADP:ATP antiporter that mediates import of ADP into the mitochondrial matrix for ATP synthesis, and export of ATP out to fuel the cell. Cycles between the cytoplasmic-open state (c-state) and the matrix-open state (m-state): operates by the alternating access mechanism with a single substrate-binding site intermittently exposed to either the cytosolic (c-state) or matrix (m-state) side of the inner mitochondrial membrane. In addition to its ADP:ATP antiporter activity, also involved in mitochondrial uncoupling and mitochondrial permeability transition pore (mPTP) activity. Plays a role in mitochondrial uncoupling by acting as a proton transporter: proton transport uncouples the proton flows via the electron transport chain and ATP synthase to reduce the efficiency of ATP production and cause mitochondrial thermogenesis. Proton transporter activity is inhibited by ADP:ATP antiporter activity, suggesting that SLC25A6/ANT3 acts as a master regulator of mitochondrial energy output by maintaining a delicate balance between ATP production (ADP:ATP antiporter activity) and thermogenesis (proton transporter activity). Proton transporter activity requires free fatty acids as cofactor, but does not transport it. Also plays a key role in mPTP opening, a non-specific pore that enables free passage of the mitochondrial membranes to solutes of up to 1.5 kDa, and which contributes to cell death. It is however unclear if SLC25A6/ANT3 constitutes a pore-forming component of mPTP or regulates it.
Protein Name Adp/Atp Translocase 3
Adp -Atp Carrier Protein 3
Adp -Atp Carrier Protein - Isoform T2
Ant 2
Adenine Nucleotide Translocator 3
Ant 3
Solute Carrier Family 25 Member 6 Cleaved Into - Adp/Atp Translocase 3 - N-Terminally Processed
Database Links Reactome: R-HSA-1268020
Reactome: R-HSA-168277
Reactome: R-HSA-180897
Reactome: R-HSA-83936
Cellular Localisation Mitochondrion Inner Membrane
Multi-Pass Membrane Protein
Membrane
The Complex Formed With Arl2bp
Arl2 And Slc25a6/Ant3 Is Expressed In Mitochondria
May Localize To Non-Mitochondrial Membranes
Alternative Antibody Names Anti-Adp/Atp Translocase 3 antibody
Anti-Adp -Atp Carrier Protein 3 antibody
Anti-Adp -Atp Carrier Protein - Isoform T2 antibody
Anti-Ant 2 antibody
Anti-Adenine Nucleotide Translocator 3 antibody
Anti-Ant 3 antibody
Anti-Solute Carrier Family 25 Member 6 Cleaved Into - Adp/Atp Translocase 3 - N-Terminally Processed antibody
Anti-SLC25A6 antibody
Anti-AAC3 antibody
Anti-ANT3 antibody
Anti-CDABP0051 antibody

Information sourced from Uniprot.org

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