| Host: | Rabbit |
| Applications: | WB/IHC/IF/ELISA |
| Reactivity: | Human/Rat/Mouse |
| Note: | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description : | Rabbit polyclonal antibody anti-ADP/ATP translocase 3 (121-170 aa) is suitable for use in Western Blot, Immunohistochemistry, Immunofluorescence and ELISA research applications. |
| Clonality : | Polyclonal |
| Conjugation: | Unconjugated |
| Isotype: | IgG |
| Formulation: | Liquid in PBS containing 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide. |
| Purification: | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Concentration: | 1 mg/mL |
| Dilution Range: | WB 1:500-1:2000 IHC 1:100-1:300 ELISA 1:40000 IF 1:50-200 |
| Storage Instruction: | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: | SLC25A6 |
| Gene ID: | 293 |
| Uniprot ID: | ADT3_HUMAN |
| Immunogen Region: | 121-170 aa |
| Specificity: | ANT3 Polyclonal Antibody detects endogenous levels of ANT3 protein. |
| Immunogen: | The antiserum was produced against synthesized peptide derived from the human SLC25A6 at the amino acid range 121-170 |
| Post Translational Modifications | Trimethylated by ANTKMT at Lys-52. |
| Function | ADP:ATP antiporter that mediates import of ADP into the mitochondrial matrix for ATP synthesis, and export of ATP out to fuel the cell. Cycles between the cytoplasmic-open state (c-state) and the matrix-open state (m-state): operates by the alternating access mechanism with a single substrate-binding site intermittently exposed to either the cytosolic (c-state) or matrix (m-state) side of the inner mitochondrial membrane. In addition to its ADP:ATP antiporter activity, also involved in mitochondrial uncoupling and mitochondrial permeability transition pore (mPTP) activity. Plays a role in mitochondrial uncoupling by acting as a proton transporter: proton transport uncouples the proton flows via the electron transport chain and ATP synthase to reduce the efficiency of ATP production and cause mitochondrial thermogenesis. Proton transporter activity is inhibited by ADP:ATP antiporter activity, suggesting that SLC25A6/ANT3 acts as a master regulator of mitochondrial energy output by maintaining a delicate balance between ATP production (ADP:ATP antiporter activity) and thermogenesis (proton transporter activity). Proton transporter activity requires free fatty acids as cofactor, but does not transport it. Also plays a key role in mPTP opening, a non-specific pore that enables free passage of the mitochondrial membranes to solutes of up to 1.5 kDa, and which contributes to cell death. It is however unclear if SLC25A6/ANT3 constitutes a pore-forming component of mPTP or regulates it. |
| Protein Name | Adp/Atp Translocase 3 Adp -Atp Carrier Protein 3 Adp -Atp Carrier Protein - Isoform T2 Ant 2 Adenine Nucleotide Translocator 3 Ant 3 Solute Carrier Family 25 Member 6 Cleaved Into - Adp/Atp Translocase 3 - N-Terminally Processed |
| Database Links | Reactome: R-HSA-1268020 Reactome: R-HSA-168277 Reactome: R-HSA-180897 Reactome: R-HSA-83936 Reactome: R-HSA-9837999 |
| Cellular Localisation | Mitochondrion Inner Membrane Multi-Pass Membrane Protein Membrane The Complex Formed With Arl2bp Arl2 And Slc25a6/Ant3 Is Expressed In Mitochondria May Localize To Non-Mitochondrial Membranes |
| Alternative Antibody Names | Anti-Adp/Atp Translocase 3 antibody Anti-Adp -Atp Carrier Protein 3 antibody Anti-Adp -Atp Carrier Protein - Isoform T2 antibody Anti-Ant 2 antibody Anti-Adenine Nucleotide Translocator 3 antibody Anti-Ant 3 antibody Anti-Solute Carrier Family 25 Member 6 Cleaved Into - Adp/Atp Translocase 3 - N-Terminally Processed antibody Anti-SLC25A6 antibody Anti-AAC3 antibody Anti-ANT3 antibody Anti-CDABP0051 antibody |
Information sourced from Uniprot.org