Anti-SLC25A4/ANT1 antibody [SRM14428] (STJ11109158)
SPECIFICATIONS
ClonalityMonoclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
ImmunogenSynthetic peptide. This information is considered to be commercially sensitive.
General Information
| Short Description | Rabbit monoclonal SLC25A4/ANT1 antibody for use in WB, IHC-P, IF, ICC, IP and ELISA in human, mouse and rat samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications | WB/IHC-P/IF/ICC/IP/ELISA |
| Host | Rabbit |
| Reactivity | Human/Mouse/Rat |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Monoclonal |
| Clone ID | SRM14428 |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Purification | Affinity purification |
| Dilution Range | WB, 1:1000-1:2000IHC-P, 1:3000-1:12000IF/ICC, 1:100-1:1000IP, 0.5 Mu g-4 Mu g antibody for 200 Mu g-400 Mu g extracts of whole cellsELISA, Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on |
| Storage Instruction | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
Target Information
| Gene Symbol | SLC25A4 |
| Gene ID | 291 |
| Uniprot ID | ADT1_HUMAN |
| Immunogen | Synthetic peptide. This information is considered to be commercially sensitive. |
Additional Info
| Tissue Specificity | Expressed in erythrocytes (at protein level). |
| Post Translational Modifications | Under cell death induction, transglutaminated by TGM2. Transglutamination leads to formation of covalent cross-links between a glutamine and the epsilon-amino group of a lysine residue, forming polymers. |
| Function | ADP:ATP antiporter that mediates import of ADP into the mitochondrial matrix for ATP synthesis, and export of ATP out to fuel the cell. Cycles between the cytoplasmic-open state (c-state) and the matrix-open state (m-state): operates by the alternating access mechanism with a single substrate-binding site intermittently exposed to either the cytosolic (c-state) or matrix (m-state) side of the inner mitochondrial membrane. In addition to its ADP:ATP antiporter activity, also involved in mitochondrial uncoupling and mitochondrial permeability transition pore (mPTP) activity. Plays a role in mitochondrial uncoupling by acting as a proton transporter: proton transport uncouples the proton flows via the electron transport chain and ATP synthase to reduce the efficiency of ATP production and cause mitochondrial thermogenesis. Proton transporter activity is inhibited by ADP:ATP antiporter activity, suggesting that SLC25A4/ANT1 acts as a master regulator of mitochondrial energy output by maintaining a delicate balance between ATP production (ADP:ATP antiporter activity) and thermogenesis (proton transporter activity). Proton transporter activity requires free fatty acids as cofactor, but does not transport it. Also plays a key role in mPTP opening, a non-specific pore that enables free passage of the mitochondrial membranes to solutes of up to 1.5 kDa, and which contributes to cell death. It is however unclear if SLC25A4/ANT1 constitutes a pore-forming component of mPTP or regulates it. Acts as a regulator of mitophagy independently of ADP:ATP antiporter activity: promotes mitophagy via interaction with TIMM44, leading to inhibit the presequence translocase TIMM23, thereby promoting stabilization of PINK1. |
| Protein Name | Adp/Atp Translocase 1Adp -Atp Carrier Protein 1Adp -Atp Carrier Protein - Heart/Skeletal Muscle Isoform T1Adenine Nucleotide Translocator 1Ant 1Solute Carrier Family 25 Member 4 |
| Database Links | Reactome: R-HSA-1268020Reactome: R-HSA-166187Reactome: R-HSA-180897Reactome: R-HSA-83936 |
| Cellular Localisation | Mitochondrion Inner MembraneMulti-Pass Membrane ProteinMembraneThe Complex Formed With Arl2bpArl2 And Slc25a4/Ant1 Is Expressed In MitochondriaMay Localize To Non-Mitochondrial Membranes |
| Alternative Antibody Names | Anti-Adp/Atp Translocase 1 antibodyAnti-Adp -Atp Carrier Protein 1 antibodyAnti-Adp -Atp Carrier Protein - Heart/Skeletal Muscle Isoform T1 antibodyAnti-Adenine Nucleotide Translocator 1 antibodyAnti-Ant 1 antibodyAnti-Solute Carrier Family 25 Member 4 antibodyAnti-SLC25A4 antibodyAnti-AAC1 antibodyAnti-ANT1 antibody |
Information sourced from Uniprot.org