• Western blot analysis of various lysates using SLC1A5 Rabbit polyclonal antibody (STJ29061) at 1:700 dilution. Secondary antibody:HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/ proteins: 25 Mu g per lane. Blocking buffer: 3 % nonfat dry milk in TBST. Detection:ECL Basic Kit. Exposuretime: 30s.

Anti-SLC1A5 antibody (154-541) (STJ29061)

SKU:
STJ29061

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Host: Rabbit
Applications: WB
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-SLC1A5 (154-541) is suitable for use in Western Blot research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.05% Proclin300, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:1000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: SLC1A5
Gene ID: 6510
Uniprot ID: AAAT_HUMAN
Immunogen Region: 154-541
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 154-541 of human SLC1A5 (NP_005619.1).
Immunogen Sequence: QPGAASAAINASVGAAGSAE NAPSKEVLDSFLDLARNIFP SNLVSAAFRSYSTTYEERNI TGTRVKVPVGQ
Tissue Specificity Placenta, lung, skeletal muscle, kidney, pancreas, and intestine. Expressed in CD34-positive hematopoietic progenitors (at protein level).
Function Sodium-coupled antiporter of neutral amino acids. In a tri-substrate transport cycle, exchanges neutral amino acids between the extracellular and intracellular compartments, coupled to the inward cotransport of at least one sodium ion. The preferred substrate is the essential amino acid L-glutamine, a precursor for biosynthesis of proteins, nucleotides and amine sugars as well as an alternative fuel for mitochondrial oxidative phosphorylation. Exchanges L-glutamine with other neutral amino acids such as L-serine, L-threonine and L-asparagine in a bidirectional way. Provides L-glutamine to proliferating stem and activated cells driving the metabolic switch toward cell differentiation. The transport cycle is usually pH-independent, with the exception of L-glutamate. Transports extracellular L-glutamate coupled to the cotransport of one proton and one sodium ion in exchange for intracellular L-glutamine counter-ion. May provide for L-glutamate uptake in glial cells regulating glutamine/glutamate cycle in the nervous system. Can transport D-amino acids. Mediates D-serine release from the retinal glia potentially affecting NMDA receptor function in retinal neurons. Displays sodium- and amino acid-dependent but uncoupled channel-like anion conductance with a preference SCN(-) >> NO3(-) > I(-) > Cl(-). Through binding of the fusogenic protein syncytin-1/ERVW-1 may mediate trophoblasts syncytialization, the spontaneous fusion of their plasma membranes, an essential process in placental development. (Microbial infection) Acts as a cell surface receptor for Feline endogenous virus RD114. (Microbial infection) Acts as a cell surface receptor for Baboon M7 endogenous virus. (Microbial infection) Acts as a cell surface receptor for type D simian retroviruses.
Protein Name Neutral Amino Acid Transporter B(0
Atb(0
Baboon M7 Virus Receptor
Rd114/Simian Type D Retrovirus Receptor
Sodium-Dependent Neutral Amino Acid Transporter Type 2
Solute Carrier Family 1 Member 5
Database Links Reactome: R-HSA-352230
Reactome: R-HSA-9013149
Reactome: R-HSA-9013406
Reactome: R-HSA-9013407
Reactome: R-HSA-9013409
Reactome: R-HSA-9013423
Cellular Localisation Cell Membrane
Multi-Pass Membrane Protein
Melanosome
Identified By Mass Spectrometry In Melanosome Fractions From Stage I To Stage Iv
Alternative Antibody Names Anti-Neutral Amino Acid Transporter B(0 antibody
Anti-Atb(0 antibody
Anti-Baboon M7 Virus Receptor antibody
Anti-Rd114/Simian Type D Retrovirus Receptor antibody
Anti-Sodium-Dependent Neutral Amino Acid Transporter Type 2 antibody
Anti-Solute Carrier Family 1 Member 5 antibody
Anti-SLC1A5 antibody
Anti-ASCT2 antibody
Anti-M7V1 antibody
Anti-RDR antibody
Anti-RDRC antibody

Information sourced from Uniprot.org

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