Anti-SLC12A6 antibody (60-190) (STJ117853)
SPECIFICATIONS
ClonalityPolyclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
General Information
| Short Description | Rabbit polyclonal SLC12A6 (60-190) antibody for use in IF, ICC and ELISA in human samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications | IF/ICC/ELISA |
| Host | Rabbit |
| Reactivity | Human |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Concentration | Lot specific |
| Purification | Affinity purification |
| Dilution Range | IF/ICC:1:50-1:200ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements. |
| Formulation | PBS with 0.02% Sodium Azide, 50% Glycerol, pH 7.3. |
| Storage Instruction | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
Target Information
| Gene Symbol | SLC12A6 |
| Gene ID | 9990 |
| Uniprot ID | S12A6_HUMAN |
| Immunogen Region | 60-190 |
| Immunogen Sequence | MSEMSGATTSLATVALDPPS DRTSHPQDVIEDLSQNSITG EHSQLLDDGHKKARNAYLNN SNYEEGDEYFDKNLALFEEE MDTRPKVSSLLNRMANYTNL TQGAKEHEEAENITEGKKKP TKTPQMGTFMG |
| Specificity | Recombinant fusion protein containing a sequence corresponding to amino acids 60-190 of human SLC12A6 (NP_598408.1). |
Additional Info
| Tissue Specificity | Expressed in brain (at protein level). Highly expressed in heart, brain and kidney. Detected at lower levels in skeletal muscle, placenta, lung and pancreas. Detected in umbilical vein endothelial cells. Isoform 2: More abundant in kidney. Isoform 5: Testis specific. |
| Post Translational Modifications | Phosphorylated, phosphorylation regulates transporter activity. Phosphorylated at Thr-991 and Thr-1048 by OXSR1/OSR1 and STK39/SPAK downstream of WNK kinases (WNK1, WNK2, WNK3 or WNK4), inhibiting the potassium-chloride cotransport activity. N-glycosylated. |
| Function | Isoform 1: Mediates electroneutral potassium-chloride cotransport when activated by cell swelling. May contribute to cell volume homeostasis in single cells. Isoform 2: Mediates electroneutral potassium-chloride cotransport when activated by cell swelling. May contribute to cell volume homeostasis in single cells (Probable). Isoform 3: Mediates electroneutral potassium-chloride cotransport when activated by cell swelling. May contribute to cell volume homeostasis in single cells (Probable). Isoform 4: Mediates electroneutral potassium-chloride cotransport when activated by cell swelling. May contribute to cell volume homeostasis in single cells (Probable). Isoform 5: Mediates electroneutral potassium-chloride cotransport when activated by cell swelling. May contribute to cell volume homeostasis in single cells (Probable). Isoform 6: Mediates electroneutral potassium-chloride cotransport when activated by cell swelling. May contribute to cell volume homeostasis in single cells (Probable). |
| Protein Name | Solute Carrier Family 12 Member 6Electroneutral Potassium-Chloride Cotransporter 3K-Cl Cotransporter 3 |
| Database Links | Reactome: R-HSA-426117Reactome: R-HSA-5619039 |
| Cellular Localisation | Cell MembraneMulti-Pass Membrane ProteinBasolateral Cell Membrane |
| Alternative Antibody Names | Anti-Solute Carrier Family 12 Member 6 antibodyAnti-Electroneutral Potassium-Chloride Cotransporter 3 antibodyAnti-K-Cl Cotransporter 3 antibodyAnti-SLC12A6 antibodyAnti-KCC3 antibody |
Information sourced from Uniprot.org