• Western blot of mouse kidney lysate showing specific immunolabeling of the ~160 kDa NCC protein phosphorylated at Thr53 in the first lane (-). Phosphospecificity is shown in the second lane (+) where immunolabeling is completely eliminated by blot treatment with lambda phosphatase ( Lambda-Ptase, 1200 units for 30 min).
  • Immunostaining of PFA perfused frozen kidney sections from WT and NCC KO mice showing specific labeling of the NCC protein phosphorylated at Thr53 (STJA0003832, red, 1:100,000) on the left and the absence of staining in the KO on the right. (Image courtesy of Lauren Miller, Ellison Lab, OHSU. )

Anti-SLC12A3 antibody (STJA0003832)

SKU:
STJA0003832-100

€568.13
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Host: Rabbit
Applications: WB/IHC
Reactivity: Human/Mouse/Rat/Guinea Pig/Hamster
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-NCC is suitable for use in Western Blot and Immunohistochemistry research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: 100 µl in 10 mM HEPES (pH 7.5) , 150 mM NaCl, 100 µg per ml BSA and 50% Glycerol.
Purification: This antibody was antigen affinity purified from pooled serum.
Dilution Range: WB 1:1000-1:6000
IHC 1:100-1:10, 000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: Slc12a3
Gene ID: 20497
Uniprot ID: S12A3_MOUSE
Immunogen: Synthetic phospho-peptide corresponding to amino acid residues surrounding Thr53 conjugated to KLH of mouse species
Tissue Specificity Expressed predominantly in kidney, including in distal tubules (at protein level). Detected at low levels in heart, lung and liver. Not detected in normal aorta, but abundantly expressed in fatty streaks and advanced atherosclerotic lesions. In atherosclerotic lesions, expressed in macrophages, smooth muscle cells and endothelial cells (at protein level).
Post Translational Modifications Ubiquitinated.ubiquitination is essential for regulation of endocytosis. Phosphorylated at Thr-53, Thr-58 and Ser-71 by OXSR1/OSR1 and STK39/SPAK downstream of WNK4, promoting its activity. Phosphorylated in response to IL18.
Function Electroneutral sodium and chloride ion cotransporter, which acts as a key mediator of sodium and chloride reabsorption in kidney distal convoluted tubules. Also acts as a receptor for the pro-inflammatory cytokine IL18, thereby contributing to IL18-induced cytokine production, including IFNG, IL6, IL18 and CCL2. May act either independently of IL18R1, or in a complex with IL18R1.
Protein Name Solute Carrier Family 12 Member 3
Na-Cl Symporter
Thiazide-Sensitive Sodium-Chloride Cotransporter
Database Links Reactome: R-MMU-426117
Cellular Localisation Cell Membrane
Multi-Pass Membrane Protein
Apical Cell Membrane
Alternative Antibody Names Anti-Solute Carrier Family 12 Member 3 antibody
Anti-Na-Cl Symporter antibody
Anti-Thiazide-Sensitive Sodium-Chloride Cotransporter antibody
Anti-Slc12a3 antibody
Anti-Ncc antibody
Anti-Tsc antibody

Information sourced from Uniprot.org

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