Anti-SLC12A3 antibody (945-995 aa) (STJ13102281)
SPECIFICATIONS
ClonalityPolyclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
ImmunogenA synthetic peptide from aa region 945-995 of human SLC12A3 conjugated to blue carrier protein was used as the antigen.
General Information
| Short Description | Rabbit polyclonal anti-SLC12A3 (945-995 aa) for use in IHC and WB in Human samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications | IHC/WB |
| Host | Rabbit |
| Reactivity | Human |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Purification | IgG purified |
| Dilution Range | IHC, WB (confirmed by recombinant protein). A dilution of 1: 200 to 1: 2000 is recommended. The optimal dilution should be determined by the end user. Not yet tested in other applications. |
| Formulation | Lyophilised |
| Storage Instruction | Maintain the lyophilised/reconstituted antibodies frozen at-20°C for long term storage and refrigerated at 2-8°C for a shorter term. When reconstituting, Glycerol (1:1) may be added for an additional stability. Avoid freeze and thaw cycles. |
Target Information
| Gene Symbol | SLC12A3 |
| Gene ID | 6559 |
| Uniprot ID | S12A3_HUMAN |
| Immunogen | A synthetic peptide from aa region 945-995 of human SLC12A3 conjugated to blue carrier protein was used as the antigen. |
| Immunogen Region | 945-995 aa |
| Specificity | Specific for SLC12A3. |
Additional Info
| Post Translational Modifications | Ubiquitinated.ubiquitination is essential for regulation of endocytosis. The BCR(KLHL3) complex was initially identified as a candidate ubiquitin ligase for SLC12A3. However, it was later shown that it is not the case. Phosphorylated at Thr-46, Thr-55, Thr-60 and Ser-91 by OXSR1/OSR1 and STK39/SPAK downstream of WNK4, promoting its activity. Phosphorylated in response to IL18. |
| Function | Electroneutral sodium and chloride ion cotransporter, which acts as a key mediator of sodium and chloride reabsorption in kidney distal convoluted tubules. Also acts as a receptor for the pro-inflammatory cytokine IL18, thereby contributing to IL18-induced cytokine production, including IFNG, IL6, IL18 and CCL2. May act either independently of IL18R1, or in a complex with IL18R1. |
| Protein Name | Solute Carrier Family 12 Member 3Na-Cl CotransporterNccNa-Cl SymporterThiazide-Sensitive Sodium-Chloride Cotransporter |
| Database Links | Reactome: R-HSA-426117Reactome: R-HSA-5619087 |
| Cellular Localisation | Cell MembraneMulti-Pass Membrane ProteinApical Cell Membrane |
| Alternative Antibody Names | Anti-Solute Carrier Family 12 Member 3 antibodyAnti-Na-Cl Cotransporter antibodyAnti-Ncc antibodyAnti-Na-Cl Symporter antibodyAnti-Thiazide-Sensitive Sodium-Chloride Cotransporter antibodyAnti-SLC12A3 antibodyAnti-NCC antibodyAnti-TSC antibody |
Information sourced from Uniprot.org