• STJ72112 Immunofluorescence analysis of paraformaldehyde fixed HeLa cells, permeabilized with 0. 15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml) , showing cytoplasmic/vesicle and membrane staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml).
  • STJ72112 Flow cytometric analysis of paraformaldehyde fixed HeLa cells (blue line) , permeabilized with 0. 5% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (1ug/ml). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.

Anti-SLC12A2 antibody (Internal) (STJ72112)

SKU:
STJ72112-100

£303.50
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Host: Goat
Applications: Pep-ELISA/IHC/IF/FC
Reactivity: Human/Mouse/Rat/Dog/Cow
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Goat polyclonal antibody anti-SLC12A2 (Internal) is suitable for use in ELISA, Immunohistochemistry, Immunofluorescence and Flow Cytometry research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. NA
Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Concentration: 0.5 mg/mL
Dilution Range: IHC-3.75µg/ml
IF-Strong expression of the protein seen in the cytoplasm/vesicles of HeLa cells. 10µg/ml
ELISA-antibody detection limit dilution 1:64000.
Storage Instruction: Store at-20°C on receipt and minimise freeze-thaw cycles.
Gene Symbol: SLC12A2
Gene ID: 6558
Uniprot ID: S12A2_HUMAN
Immunogen Region: Internal
Accession Number: NP_001037.1; NP_001243390.1
Immunogen Sequence: NALQHSIRLSGVEDH
Post Translational Modifications Phosphorylated at Thr-203, Thr-207 and Thr-212 by OXSR1/OSR1 and STK39/SPAK downstream of WNK kinases (WNK1, WNK2, WNK3 or WNK4), promoting its activity.
Function Cation-chloride cotransporter which mediates the electroneutral transport of chloride, potassium and/or sodium ions across the membrane. Plays a vital role in the regulation of ionic balance and cell volume.
Protein Name Solute Carrier Family 12 Member 2
Basolateral Na-K-Cl Symporter
Bumetanide-Sensitive Sodium-(Potassium-Chloride Cotransporter 2
Database Links Reactome: R-HSA-426117
Cellular Localisation Basolateral Cell Membrane
Multi-Pass Membrane Protein
Alternative Antibody Names Anti-Solute Carrier Family 12 Member 2 antibody
Anti-Basolateral Na-K-Cl Symporter antibody
Anti-Bumetanide-Sensitive Sodium-(Potassium-Chloride Cotransporter 2 antibody
Anti-SLC12A2 antibody
Anti-NKCC1 antibody

Information sourced from Uniprot.org

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