| Host: |
Rabbit |
| Applications: |
WB |
| Reactivity: |
Mouse/Rat |
| Note: |
STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description: |
Rabbit polyclonal antibody anti-SLC12A1 (50-150) is suitable for use in Western Blot research applications. |
| Clonality: |
Polyclonal |
| Conjugation: |
Unconjugated |
| Isotype: |
IgG |
| Formulation: |
PBS with 0.01% Thimerosal, 50% Glycerol, pH7.3. |
| Purification: |
Affinity purification |
| Dilution Range: |
WB 1:500-1:2000 |
| Storage Instruction: |
Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: |
SLC12A1 |
| Gene ID: |
6557 |
| Uniprot ID: |
S12A1_HUMAN |
| Immunogen Region: |
50-150 |
| Immunogen: |
A synthetic peptide corresponding to a sequence within amino acids 50-150 of human SLC12A1 (NP_000329.2). |
| Immunogen Sequence: |
FGDEAQKRLRISFRPGNQEC YDNFLQSGETAKTDASFHAY DSHTNTYYLQTFGHNTMDAV PKIEYYRNTGSISGPKVNRP SLLEIHEQLAKNVAVTPSSA D |
| Tissue Specificity | Kidney.localizes to the thick ascending limbs (at protein level). |
| Post Translational Modifications | Phosphorylated at Ser-91, Thr-100 and Thr-105 by OXSR1/OSR1 and STK39/SPAK downstream of WNK kinases (WNK1, WNK2, WNK3 or WNK4), promoting its activity. |
| Function | Renal sodium, potassium and chloride ion cotransporter that mediates the transepithelial NaCl reabsorption in the thick ascending limb and plays an essential role in the urinary concentration and volume regulation. Electrically silent transporter system. |
| Protein Name | Solute Carrier Family 12 Member 1Bumetanide-Sensitive Sodium-(Potassium-Chloride Cotransporter 2Kidney-Specific Na-K-Cl Symporter |
| Database Links | Reactome: R-HSA-426117Reactome: R-HSA-5619104 |
| Cellular Localisation | Apical Cell MembraneMulti-Pass Membrane Protein |
| Alternative Antibody Names | Anti-Solute Carrier Family 12 Member 1 antibodyAnti-Bumetanide-Sensitive Sodium-(Potassium-Chloride Cotransporter 2 antibodyAnti-Kidney-Specific Na-K-Cl Symporter antibodyAnti-SLC12A1 antibodyAnti-NKCC2 antibody |
Information sourced from Uniprot.org
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
