• Western blot analysis of extracts of various cell lines, using SKIV2L2 antibody (STJ27546) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 60s.
  • Immunohistochemistry of paraffin-embedded human breast cancer using SKIV2L2 antibody (STJ27546) at dilution of 1:100 (40x lens).
  • Immunohistochemistry of paraffin-embedded mouse testis using SKIV2L2 antibody (STJ27546) at dilution of 1:100 (40x lens).
  • Immunoprecipitation analysis of 300ug extracts of 293T cells using 3ug SKIV2L2 antibody (STJ27546). Western blot was performed from the immunoprecipitate using SKIV2L2 antibody (STJ27546) at a dilition of 1:500.

Anti-SKIV2L2 antibody (1-304) (STJ27546)

SKU:
STJ27546

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Host: Rabbit
Applications: WB, IHC, IP
Reactivity: Human, Mouse
Note: FOR RESEARCH USE ONLY (RUO).
Short Description: Rabbit polyclonal antibody anti-SKIV2L2 (1-304) is suitable for use in Western Blot, Immunohistochemistry and Immunoprecipitation.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS containing 0.02% Sodium Azide, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
IHC 1:50-1:200
IP 1:50-1:200
Storage Instruction: Store in a freezer at-20°C and avoid freeze-thaw cycles.
Gene Symbol: MTREX
Gene ID: 23517
Uniprot ID: MTREX_HUMAN
Immunogen Region: 1-304
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-304 of human SKIV2L2 (NP_056175.3).
Function Catalyzes the ATP-dependent unwinding of RNA duplexes with a single-stranded 3' RNA extension. Central subunit of many protein complexes, namely TRAMP-like, nuclear exosome targeting (NEXT) and poly(A) tail exosome targeting (PAXT). NEXT functions as an RNA exosome cofactor that directs a subset of non-coding short-lived RNAs for exosomal degradation. NEXT is involved in surveillance and turnover of aberrant transcripts and non-coding RNAs. PAXT directs a subset of long and polyadenylated poly(A) RNAs for exosomal degradation. The RNA exosome is fundamental for the degradation of RNA in eukaryotic nuclei. Substrate targeting is facilitated by its cofactor ZCCHC8, which links to RNA-binding protein adapters. Associated with the RNA exosome complex and involved in the 3'-processing of the 7S pre-RNA to the mature 5.8S rRNA. May be involved in pre-mRNA splicing. In the context of NEXT complex can also in vitro unwind DNA:RNA heteroduplexes with a 3' poly (A) RNA tracking strand. Can promote unwinding and degradation of structured RNA substrates when associated with the nuclear exosome and its cofactors. Can displace a DNA strand while translocating on RNA to ultimately degrade the RNA within a DNA/RNA heteroduplex. Plays a role in DNA damage response.
Protein Name Exosome Rna Helicase Mtr4
Atp-Dependent Rna Helicase Dob1
Atp-Dependent Rna Helicase Skiv2l2
Superkiller Viralicidic Activity 2-Like 2
Tramp-Like Complex Helicase
Database Links Reactome: R-HSA-6791226
Reactome: R-HSA-72163
Cellular Localisation Nucleus
Nucleoplasm
Nucleolus
Nucleus Speckle
Alternative Antibody Names Anti-Exosome Rna Helicase Mtr4 antibody
Anti-Atp-Dependent Rna Helicase Dob1 antibody
Anti-Atp-Dependent Rna Helicase Skiv2l2 antibody
Anti-Superkiller Viralicidic Activity 2-Like 2 antibody
Anti-Tramp-Like Complex Helicase antibody
Anti-MTREX antibody
Anti-DOB1 antibody
Anti-KIAA0052 antibody
Anti-MTR4 antibody
Anti-SKIV2L2 antibody

Information sourced from Uniprot.org

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