• Immunofluorescence analysis of HeLa cells using SKAP2 antibody (STJ28518). Blue: DAPI for nuclear staining.
  • Western blot analysis of extracts of mouse lung, using SKAP2 antibody (STJ28518) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.

Anti-SKAP2 antibody (1-120) (STJ28518)

SKU:
STJ28518

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Host: Rabbit
Applications: WB/IF
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-SKAP2 (1-120) is suitable for use in Western Blot and Immunofluorescence research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
IF/ICC 1:50-1:100
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: SKAP2
Gene ID: 8935
Uniprot ID: SKAP2_HUMAN
Immunogen Region: 1-120
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-120 of human SKAP2 (NP_003921.2).
Immunogen Sequence: MPNPSSTSSPYPLPEEIRNL LADVETFVADILKGENLSKK AKEKRESLIKKIKDVKSIYL QEFQDKGDAEDGEEYDDPFA GPPDTISLASERYDKDDEAP SDGAQFPPIAAQDLPFVLKA
Tissue Specificity Ubiquitously expressed. Present in platelets (at protein level).
Post Translational Modifications Phosphorylated in resting platelets. Phosphorylated by FYN on Tyr-261 upon T-cell activation (Probable). Dephosphorylated on Tyr-75 by PTPN22.
Function May be involved in B-cell and macrophage adhesion processes. In B-cells, may act by coupling the B-cell receptor (BCR) to integrin activation. May play a role in src signaling pathway.
Protein Name Src Kinase-Associated Phosphoprotein 2
Pyk2/Raftk-Associated Protein
Retinoic Acid-Induced Protein 70
Skap55 Homolog
Skap-55hom
Skap-Hom
Src Family-Associated Phosphoprotein 2
Src Kinase-Associated Phosphoprotein 55-Related Protein
Src-Associated Adapter Protein With Ph And Sh3 Domains
Database Links Reactome: R-HSA-391160
Cellular Localisation Cytoplasm
Alternative Antibody Names Anti-Src Kinase-Associated Phosphoprotein 2 antibody
Anti-Pyk2/Raftk-Associated Protein antibody
Anti-Retinoic Acid-Induced Protein 70 antibody
Anti-Skap55 Homolog antibody
Anti-Skap-55hom antibody
Anti-Skap-Hom antibody
Anti-Src Family-Associated Phosphoprotein 2 antibody
Anti-Src Kinase-Associated Phosphoprotein 55-Related Protein antibody
Anti-Src-Associated Adapter Protein With Ph And Sh3 Domains antibody
Anti-SKAP2 antibody
Anti-PRAP antibody
Anti-RA70 antibody
Anti-SAPS antibody
Anti-SCAP2 antibody
Anti-SKAP55R antibody

Information sourced from Uniprot.org

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