| Host: |
Rabbit |
| Applications: |
WB |
| Reactivity: |
Mouse |
| Note: |
STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description: |
Rabbit polyclonal antibody anti-SIX1 (130-284) is suitable for use in Western Blot research applications. |
| Clonality: |
Polyclonal |
| Conjugation: |
Unconjugated |
| Isotype: |
IgG |
| Formulation: |
PBS with 0.01% Thimerosal, 50% Glycerol, pH7.3. |
| Purification: |
Affinity purification |
| Dilution Range: |
WB 1:500-1:1000 |
| Storage Instruction: |
Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: |
SIX1 |
| Gene ID: |
6495 |
| Uniprot ID: |
SIX1_HUMAN |
| Immunogen Region: |
130-284 |
| Immunogen: |
Recombinant fusion protein containing a sequence corresponding to amino acids 130-284 of human SIX1 (NP_005973.1). |
| Immunogen Sequence: |
CFKEKSRGVLREWYAHNPYP SPREKRELAEATGLTTTQVS NWFKNRRQRDRAAEAKEREN TENNNSSSNKQNQLSPLEGG KPLMSSSEEEFSPPQSPDQN SVLLLQGNMGHARSSNYSLP GLTASQPSHGLQTHQHQLQD S |
| Tissue Specificity | Specifically expressed in skeletal muscle. |
| Post Translational Modifications | Phosphorylated during interphase.becomes hyperphosphorylated during mitosis. Hyperphosphorylation impairs binding to promoter elements. Ubiquitinated by the anaphase promoting complex (APC), leading to its proteasomal degradation. |
| Function | Transcription factor that is involved in the regulation of cell proliferation, apoptosis and embryonic development. Plays an important role in the development of several organs, including kidney, muscle and inner ear. Depending on context, functions as transcriptional repressor or activator. Lacks an activation domain, and requires interaction with EYA family members for transcription activation. Mediates nuclear translocation of EYA1 and EYA2. Binds the 5'-TCAAGAGTTNC-3' motif present in the MEF3 element in the MYOG promoter and CIDEA enhancer. Regulates the expression of numerous genes, including MYC, CCND1 and EZR. Acts as activator of the IGFBP5 promoter, probably coactivated by EYA2. Repression of precursor cell proliferation in myoblasts is switched to activation through recruitment of EYA3 to the SIX1-DACH1 complex. During myogenesis, seems to act together with EYA2 and DACH2. Regulates the expression of CCNA1. Promotes brown adipocyte differentiation. |
| Protein Name | Homeobox Protein Six1Sine Oculis Homeobox Homolog 1 |
| Cellular Localisation | NucleusCytoplasm |
| Alternative Antibody Names | Anti-Homeobox Protein Six1 antibodyAnti-Sine Oculis Homeobox Homolog 1 antibodyAnti-SIX1 antibody |
Information sourced from Uniprot.org
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
