Anti-SIRT5 antibody [2F1] (STJA0032633)
SPECIFICATIONS
ClonalityMonoclonal
HostMouse
ConjugationUnconjugated
IsotypeIgG1
ImmunogenFull length human recombinant protein of human SIRT5 produced in 293T cell.
General Information
| Short Description | Mouse monoclonal anti-SIRT5 for use in WB and IHC-P in Human, Mouse and Rat samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications | WB/IHC-P |
| Host | Mouse |
| Reactivity | Human/Mouse/Rat |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Monoclonal |
| Clone ID | 2F1 |
| Isotype | IgG1 |
| Conjugation | Unconjugated |
| Concentration | 0.24 mg/mL |
| Purification | Affinity Purified |
| Dilution Range | WB 1:500-1:1000IHC 1:50-1:100 |
| Formulation | Liquid in PBS containing 50% glycerol, 1% BSA and 0.02% sodium azide, pH 7.3. |
| Storage Instruction | Store at 4°C short term. Aliquot and store at-20°C long term. Avoid freeze/thaw cycles. |
Target Information
| Gene Symbol | SIRT5 |
| Gene ID | 23408 |
| Uniprot ID | SIR5_HUMAN |
| Immunogen | Full length human recombinant protein of human SIRT5 produced in 293T cell. |
Additional Info
| Tissue Specificity | Widely expressed. |
| Function | NAD-dependent lysine demalonylase, desuccinylase and deglutarylase that specifically removes malonyl, succinyl and glutaryl groups on target proteins. Activates CPS1 and contributes to the regulation of blood ammonia levels during prolonged fasting: acts by mediating desuccinylation and deglutarylation of CPS1, thereby increasing CPS1 activity in response to elevated NAD levels during fasting. Activates SOD1 by mediating its desuccinylation, leading to reduced reactive oxygen species. Activates SHMT2 by mediating its desuccinylation. Modulates ketogenesis through the desuccinylation and activation of HMGCS2. Has weak NAD-dependent protein deacetylase activity.however this activity may not be physiologically relevant in vivo. Can deacetylate cytochrome c (CYCS) and a number of other proteins in vitro such as UOX. |
| Protein Name | Nad-Dependent Protein Deacylase Sirtuin-5 - MitochondrialRegulatory Protein Sir2 Homolog 5Sir2-Like Protein 5 |
| Database Links | Reactome: R-HSA-2151201 |
| Cellular Localisation | Mitochondrion MatrixMitochondrion Intermembrane SpaceCytoplasmCytosolNucleusMainly MitochondrialAlso Present ExtramitochondriallyWith A Fraction Present In The Cytosol And Very Small Amounts Also Detected In The NucleusIsoform 1: CytoplasmMitochondrionIsoform 2: Mitochondrion |
| Alternative Antibody Names | Anti-Nad-Dependent Protein Deacylase Sirtuin-5 - Mitochondrial antibodyAnti-Regulatory Protein Sir2 Homolog 5 antibodyAnti-Sir2-Like Protein 5 antibodyAnti-SIRT5 antibodyAnti-SIR2L5 antibody |
Information sourced from Uniprot.org