Anti-SIRT4 antibody (C-Term) (STJ70513)
SPECIFICATIONS
ClonalityPolyclonal
HostGoat
ConjugationUnconjugated
IsotypeIgG
General Information
| Short Description | Goat polyclonal antibody anti-SIRT4 (C-Term) is suitable for use in ELISA, Western Blot and Immunohistochemistry research applications. |
| Applications | Pep-ELISA/WB/IHC |
| Host | Goat |
| Reactivity | Human/Mouse/Rat/Dog/Pig/Cow |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Concentration | 0.5 mg/mL |
| Purification | Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. |
| Dilution Range | Peptide ELISA: antibody detection limit dilution 1:16000.WB: Approx. 37kDa band observed in lysates of cell lines U937 and U251, and approx. 38kDa in lysates of cell lines K562, MCF7 and U2OS and in preliminary testing of Rat Kidney lysate (calc |
| Formulation | 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. NA |
| Storage Instruction | Store at-20°C on receipt and minimise freeze-thaw cycles. |
Target Information
| Gene Symbol | SIRT4 |
| Gene ID | 23409 |
| Uniprot ID | SIR4_HUMAN |
| Accession Number | NP_036372.1 |
| Immunogen Region | C-Term |
| Immunogen Sequence | SRCGELLPLIDPC |
Additional Info
| Function | Acts as a NAD-dependent protein lipoamidase, biotinylase, deacetylase and ADP-ribosyl transferase. Catalyzes more efficiently removal of lipoyl- and biotinyl- than acetyl-lysine modifications. Inhibits the pyruvate dehydrogenase complex (PDH) activity via the enzymatic hydrolysis of the lipoamide cofactor from the E2 component, DLAT, in a phosphorylation-independent manner. Catalyzes the transfer of ADP-ribosyl groups onto target proteins, including mitochondrial GLUD1, inhibiting GLUD1 enzyme activity. Acts as a negative regulator of mitochondrial glutamine metabolism by mediating mono ADP-ribosylation of GLUD1: expressed in response to DNA damage and negatively regulates anaplerosis by inhibiting GLUD1, leading to block metabolism of glutamine into tricarboxylic acid cycle and promoting cell cycle arrest. In response to mTORC1 signal, SIRT4 expression is repressed, promoting anaplerosis and cell proliferation. Acts as a tumor suppressor. Also acts as a NAD-dependent protein deacetylase: mediates deacetylation of 'Lys-471' of MLYCD, inhibiting its activity, thereby acting as a regulator of lipid homeostasis. Does not seem to deacetylate PC. Controls fatty acid oxidation by inhibiting PPARA transcriptional activation. Impairs SIRT1-PPARA interaction probably through the regulation of NAD(+) levels. Down-regulates insulin secretion. |
| Protein Name | Nad-Dependent Protein Lipoamidase Sirtuin-4 - MitochondrialNad-Dependent Adp-Ribosyltransferase Sirtuin-4Nad-Dependent Protein Biotinylase Sirtuin-4Nad-Dependent Protein Deacetylase Sirtuin-4Regulatory Protein Sir2 Homolog 4Sir2-Like Protein 4 |
| Database Links | Reactome: R-HSA-204174Reactome: R-HSA-2151201 |
| Cellular Localisation | Mitochondrion Matrix |
| Alternative Antibody Names | Anti-Nad-Dependent Protein Lipoamidase Sirtuin-4 - Mitochondrial antibodyAnti-Nad-Dependent Adp-Ribosyltransferase Sirtuin-4 antibodyAnti-Nad-Dependent Protein Biotinylase Sirtuin-4 antibodyAnti-Nad-Dependent Protein Deacetylase Sirtuin-4 antibodyAnti-Regulatory Protein Sir2 Homolog 4 antibodyAnti-Sir2-Like Protein 4 antibodyAnti-SIRT4 antibodyAnti-SIR2L4 antibody |
Information sourced from Uniprot.org