| Host: |
Rabbit |
| Applications: |
WB/IHC |
| Reactivity: |
Human/Mouse/Rat |
| Note: |
STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description: |
Rabbit polyclonal antibody anti-SIRT4 (29-314) is suitable for use in Western Blot and Immunohistochemistry research applications. |
| Clonality: |
Polyclonal |
| Conjugation: |
Unconjugated |
| Isotype: |
IgG |
| Formulation: |
PBS with 0.05% Proclin300, 50% Glycerol, pH7.3. |
| Purification: |
Affinity purification |
| Dilution Range: |
WB 1:500-1:2000IHC-P 1:50-1:200 |
| Storage Instruction: |
Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: |
SIRT4 |
| Gene ID: |
23409 |
| Uniprot ID: |
SIR4_HUMAN |
| Immunogen Region: |
29-314 |
| Immunogen: |
Recombinant fusion protein containing a sequence corresponding to amino acids 29-314 of human SIRT4 (NP_036372.1). |
| Immunogen Sequence: |
IGLFVPASPPLDPEKVKELQ RFITLSKRLLVMTGAGISTE SGIPDYRSEKVGLYARTDRR PIQHGDFVRSAPIRQRYWAR NFVGWPQFSSHQPNPAHWAL STWEKLGKLYWLVTQNVDAL HTKAGSRRLTELHGCMDRVL CLDCGEQTPRGVLQERFQVL NPTWSAEAHGLAPDGDVFLS EEQVRSFQVPTCVQCGGHLK PDVVFFGDTVNPDKVDFVHK RVKEADSLLVVGSSLQVYS |
| Tissue Specificity | Detected in vascular smooth muscle and striated muscle. Detected in insulin-producing beta-cells in pancreas islets of Langerhans (at protein level). Widely expressed. Weakly expressed in leukocytes and fetal thymus. |
| Function | Acts as NAD-dependent protein lipoamidase, biotinylase, deacetylase and ADP-ribosyl transferase. Catalyzes more efficiently removal of lipoyl- and biotinyl- than acetyl-lysine modifications. Inhibits the pyruvate dehydrogenase complex (PDH) activity via the enzymatic hydrolysis of the lipoamide cofactor from the E2 component, DLAT, in a phosphorylation-independent manner. Catalyzes the transfer of ADP-ribosyl groups onto target proteins, including mitochondrial GLUD1, inhibiting GLUD1 enzyme activity. Acts as a negative regulator of mitochondrial glutamine metabolism by mediating mono ADP-ribosylation of GLUD1: expressed in response to DNA damage and negatively regulates anaplerosis by inhibiting GLUD1, leading to block metabolism of glutamine into tricarboxylic acid cycle and promoting cell cycle arrest. In response to mTORC1 signal, SIRT4 expression is repressed, promoting anaplerosis and cell proliferation. Acts as a tumor suppressor. Also acts as a NAD-dependent protein deacetylase: mediates deacetylation of 'Lys-471' of MLYCD, inhibiting its activity, thereby acting as a regulator of lipid homeostasis. Does not seem to deacetylate PC. Controls fatty acid oxidation by inhibiting PPARA transcriptional activation. Impairs SIRT1-PPARA interaction probably through the regulation of NAD(+) levels. Down-regulates insulin secretion. |
| Protein Name | Nad-Dependent Protein Lipoamidase Sirtuin-4 - MitochondrialNad-Dependent Adp-Ribosyltransferase Sirtuin-4Nad-Dependent Protein Biotinylase Sirtuin-4Nad-Dependent Protein Deacetylase Sirtuin-4Regulatory Protein Sir2 Homolog 4Sir2-Like Protein 4 |
| Database Links | Reactome: R-HSA-2151201 |
| Cellular Localisation | Mitochondrion Matrix |
| Alternative Antibody Names | Anti-Nad-Dependent Protein Lipoamidase Sirtuin-4 - Mitochondrial antibodyAnti-Nad-Dependent Adp-Ribosyltransferase Sirtuin-4 antibodyAnti-Nad-Dependent Protein Biotinylase Sirtuin-4 antibodyAnti-Nad-Dependent Protein Deacetylase Sirtuin-4 antibodyAnti-Regulatory Protein Sir2 Homolog 4 antibodyAnti-Sir2-Like Protein 4 antibodyAnti-SIRT4 antibodyAnti-SIR2L4 antibody |
Information sourced from Uniprot.org
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
