| Host: |
Rabbit |
| Applications: |
WB/ELISA |
| Reactivity: |
Human/Mouse |
| Note: |
STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description: |
Rabbit polyclonal antibody anti-NAD-dependent protein deacetylase sirtuin-3, mitochondrial (350-399 aa) is suitable for use in Western Blot and ELISA research applications. |
| Clonality: |
Polyclonal |
| Conjugation: |
Unconjugated |
| Isotype: |
IgG |
| Formulation: |
Liquid in PBS containing 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide. |
| Purification: |
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Concentration: |
1 mg/mL |
| Dilution Range: |
WB 1:500-1:2000ELISA 1:5000 |
| Storage Instruction: |
Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: |
SIRT3 |
| Gene ID: |
23410 |
| Uniprot ID: |
SIR3_HUMAN |
| Immunogen Region: |
350-399 aa |
| Specificity: |
SIRT3 Polyclonal Antibody detects endogenous levels of SIRT3 protein. |
| Immunogen: |
The antiserum was produced against synthesized peptide derived from the human SIRT3 at the amino acid range 350-399 |
| Post Translational Modifications | Processed by mitochondrial processing peptidase (MPP) to give a 28 kDa product. Such processing is probably essential for its enzymatic activity. |
| Function | NAD-dependent protein deacetylase. Activates or deactivates mitochondrial target proteins by deacetylating key lysine residues. Known targets include ACSS1, IDH, GDH, SOD2, PDHA1, LCAD, SDHA and the ATP synthase subunit ATP5PO. Contributes to the regulation of the cellular energy metabolism. Important for regulating tissue-specific ATP levels. In response to metabolic stress, deacetylates transcription factor FOXO3 and recruits FOXO3 and mitochondrial RNA polymerase POLRMT to mtDNA to promote mtDNA transcription. Acts as a regulator of ceramide metabolism by mediating deacetylation of ceramide synthases CERS1, CERS2 and CERS6, thereby increasing their activity and promoting mitochondrial ceramide accumulation. Regulates hepatic lipogenesis. Uses NAD(+) substrate imported by SLC25A47, triggering downstream activation of PRKAA1/AMPK-alpha signaling cascade that ultimately downregulates sterol regulatory element-binding protein (SREBP) transcriptional activities and ATP-consuming lipogenesis to restore cellular energy balance. |
| Protein Name | Nad-Dependent Protein Deacetylase Sirtuin-3 - MitochondrialHsirt3Regulatory Protein Sir2 Homolog 3Sir2-Like Protein 3 |
| Database Links | Reactome: R-HSA-2151201Reactome: R-HSA-9615017Reactome: R-HSA-9617629 |
| Cellular Localisation | Mitochondrion Matrix |
| Alternative Antibody Names | Anti-Nad-Dependent Protein Deacetylase Sirtuin-3 - Mitochondrial antibodyAnti-Hsirt3 antibodyAnti-Regulatory Protein Sir2 Homolog 3 antibodyAnti-Sir2-Like Protein 3 antibodyAnti-SIRT3 antibodyAnti-SIR2L3 antibody |
Information sourced from Uniprot.org
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
![Immunofluorescence analysis of NIH/3T3 cells using [KO Validated] SIRT3 Rabbit polyclonal antibody (STJ11102849) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.](https://cdn11.bigcommerce.com/s-zso2xnchw9/images/stencil/300x300/products/163792/364538/STJ11102849_1__74784.1713129638.jpg?c=1 "Immunofluorescence analysis of NIH/3T3 cells using [KO Validated] SIRT3 Rabbit polyclonal antibody (STJ11102849) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.")
