• Western blot analysis of extracts of HepG2 cells, using SIRT3 antibody (STJ28285) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 180s.
  • Western blot analysis of extracts of mouse liver, using SIRT3 antibody (STJ28285) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.
  • Western blot analysis of extracts of various cell lines, using SIRT3 antibody (STJ28285) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.
  • Western blot analysis of extracts from wild type (WT) and SIRT3 knockout (KO) 293T cells, using SIRT3 antibody (STJ28285) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.
  • Immunohistochemistry analysis of paraffin-embedded rat kidney using SIRT3 antibody (STJ28285) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of paraffin-embedded human thyroid cancer using SIRT3 antibody (STJ28285) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of paraffin-embedded mouse kidney using SIRT3 antibody (STJ28285) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Immunofluorescence analysis of NIH/3T3 cells using SIRT3 rabbit polyclonal antibody (STJ28285) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

Anti-SIRT3 antibody (299-399) (STJ28285)

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STJ28285

$174.83 - $863.33
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Host: Rabbit
Applications: WB/IHC/IF
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-SIRT3 (299-399) is suitable for use in Western Blot, Immunohistochemistry and Immunofluorescence research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.05% Proclin300, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:1000
IHC-P 1:20-1:50
IF/ICC 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: SIRT3
Gene ID: 23410
Uniprot ID: SIR3_HUMAN
Immunogen Region: 299-399
Immunogen: A synthetic peptide corresponding to a sequence within amino acids 299-399 of human SIRT3 (NP_036371.1).
Immunogen Sequence: PQRFLLHVVDFPMADLLLIL GTSLEVEPFASLTEAVRSSV PRLLINRDLVGPLAWHPRSR DVAQLGDVVHGVESLVELLG WTEEMRDLVQRETGKLDGPD K
Tissue Specificity Widely expressed.
Post Translational Modifications Processed by mitochondrial processing peptidase (MPP) to give a 28 kDa product. Such processing is probably essential for its enzymatic activity.
Function NAD-dependent protein deacetylase. Activates or deactivates mitochondrial target proteins by deacetylating key lysine residues. Known targets include ACSS1, IDH, GDH, SOD2, PDHA1, LCAD, SDHA and the ATP synthase subunit ATP5PO. Contributes to the regulation of the cellular energy metabolism. Important for regulating tissue-specific ATP levels. In response to metabolic stress, deacetylates transcription factor FOXO3 and recruits FOXO3 and mitochondrial RNA polymerase POLRMT to mtDNA to promote mtDNA transcription. Acts as a regulator of ceramide metabolism by mediating deacetylation of ceramide synthases CERS1, CERS2 and CERS6, thereby increasing their activity and promoting mitochondrial ceramide accumulation. Regulates hepatic lipogenesis. Uses NAD(+) substrate imported by SLC25A47, triggering downstream activation of PRKAA1/AMPK-alpha signaling cascade that ultimately downregulates sterol regulatory element-binding protein (SREBP) transcriptional activities and ATP-consuming lipogenesis to restore cellular energy balance.
Protein Name Nad-Dependent Protein Deacetylase Sirtuin-3 - Mitochondrial
Hsirt3
Regulatory Protein Sir2 Homolog 3
Sir2-Like Protein 3
Database Links Reactome: R-HSA-2151201
Reactome: R-HSA-9615017
Reactome: R-HSA-9617629
Cellular Localisation Mitochondrion Matrix
Alternative Antibody Names Anti-Nad-Dependent Protein Deacetylase Sirtuin-3 - Mitochondrial antibody
Anti-Hsirt3 antibody
Anti-Regulatory Protein Sir2 Homolog 3 antibody
Anti-Sir2-Like Protein 3 antibody
Anti-SIRT3 antibody
Anti-SIR2L3 antibody

Information sourced from Uniprot.org

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