Anti-SHTN1 antibody (STJA0004299)

SKU:
STJA0004299

Current Stock:
Host: Rabbit
Applications: WB/IHC/ELISA
Reactivity: Human
Note: FOR SCIENTIFIC EDUCATIONAL RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR OTHER MEDICAL APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-Shootin-1 is suitable for use in Western Blot, Immunohistochemistry and ELISA research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.05% NaN3 and 40% Glycerol, pH7.4
Purification: Antigen affinity purification
Concentration: 0.78 mg/mL
Dilution Range: WB 1:500-1:2000, IHC 1:50-1:100
Storage Instruction: Store at-20°C for long term, and avoid freeze-thaw cycles.
Gene Symbol: SHTN1
Gene ID: 57698
Uniprot ID: SHOT1_HUMAN
Immunogen: Synthetic peptide of human SHTN1
Post Translational Modifications Phosphorylated on Ser-101 and Ser-249 by PAK1 through a CDC42- and RAC1-dependent signaling pathway, which enhances its association with F-actin retrograde flow in filopodia and lamellipodia of axonal growth cones. Phosphorylation on Ser-101 and Ser-249 is increased by netrin-1.
Function Involved in the generation of internal asymmetric signals required for neuronal polarization and neurite outgrowth. Mediates netrin-1-induced F-actin-substrate coupling or 'clutch engagement' within the axon growth cone through activation of CDC42, RAC1 and PAK1-dependent signaling pathway, thereby converting the F-actin retrograde flow into traction forces, concomitantly with filopodium extension and axon outgrowth. Plays a role in cytoskeletal organization by regulating the subcellular localization of phosphoinositide 3-kinase (PI3K) activity at the axonal growth cone. Also plays a role in regenerative neurite outgrowth. In the developing cortex, cooperates with KIF20B to promote both the transition from the multipolar to the bipolar stage and the radial migration of cortical neurons from the ventricular zone toward the superficial layer of the neocortex. Involved in the accumulation of phosphatidylinositol 3,4,5-trisphosphate (PIP3) in the growth cone of primary hippocampal neurons.
Protein Name Shootin-1
Shootin1
Database Links Reactome: R-HSA-437239
Cellular Localisation Perikaryon
Cell Projection
Axon
Growth Cone
Cytoplasm
Cytoskeleton
Filopodium
Lamellipodium
Localizes In Multiple Growth Cones At Neurite Tips Before The Neuronal Symmetry-Breaking Step
Accumulates In Growth Cones Of A Single Nascent Axon In A Neurite Length-Dependent Manner During The Neuronal Symmetry-Breaking Step
When Absent From The Nascent Axon's Siblings
Probably Due To Competitive Transport
Prevents The Formation Of Surplus Axons
Transported Anterogradely From The Soma To The Axon Growth Cone In An Actin And Myosin-Dependent Manner And Passively Diffuses Back To The Cell Bodies
Colocalized With L1cam In Close Apposition With Actin Filaments In Filopodia And Lamellipodia Of Axonal Growth Cones In Hippocampal Neurons
Exhibits Retrograde Movements In Filopodia And Lamellopodia Of Axonal Growth Cones
Colocalized With Kif20b Along Microtubules To The Tip Of The Growing Cone In Primary Hippocampal Neurons
Recruited To The Growth Cone Of Developing Axon In A Kif20b- And Microtubule-Dependent Manner
Alternative Antibody Names Anti-Shootin-1 antibody
Anti-Shootin1 antibody
Anti-SHTN1 antibody
Anti-KIAA1598 antibody

Information sourced from Uniprot.org

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