• Western blot analysis of extracts of mouse liver, using SHMT1 antibody (STJ114363) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.
  • Western blot analysis of extracts of various cell lines, using SHMT1 antibody (STJ114363) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.
  • Immunohistochemistry analysis of paraffin-embedded rat kidney using SHMT1 antibody (STJ114363) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of paraffin-embedded mouse kidney using SHMT1 antibody (STJ114363) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Immunofluorescence analysis of U-2 OS cells using SHMT1 antibody (STJ114363) at dilution of 1:100. Blue: DAPI for nuclear staining.

Anti-SHMT1 antibody (285-444) (STJ114363)

SKU:
STJ114363

$151.88 - $705.38
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Host: Rabbit
Applications: WB/IHC/IF
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-SHMT1 (285-444) is suitable for use in Western Blot, Immunohistochemistry and Immunofluorescence research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.01% Thimerosal, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:1000
IHC-P 1:50-1:200
IF/ICC 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: SHMT1
Gene ID: 6470
Uniprot ID: GLYC_HUMAN
Immunogen Region: 285-444
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 285-444 of human SHMT1 (NP_683718.1).
Immunogen Sequence: FKVYQHQVVANCRALSEALT ELGYKIVTGGSDNHLILVDL RSKGTDGGRAEKVLEACSIA CNKNTCPGDRSALRPSGLRL GTPALTSRGLLEKDFQKVAH FIHRGIELTLQIQSDTGVRA TLKEFKERLAGDKYQAAVQA LREEVESFASLFPLPGLPDF
Function Interconversion of serine and glycine.
Protein Name Serine Hydroxymethyltransferase - Cytosolic
Shmt
Glycine Hydroxymethyltransferase
Serine Methylase
Database Links Reactome: R-HSA-196757
Reactome: R-HSA-71262
Cellular Localisation Cytoplasm
Alternative Antibody Names Anti-Serine Hydroxymethyltransferase - Cytosolic antibody
Anti-Shmt antibody
Anti-Glycine Hydroxymethyltransferase antibody
Anti-Serine Methylase antibody
Anti-SHMT1 antibody

Information sourced from Uniprot.org

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