Western blot analysis of extracts of mouse liver, using SHMT1 antibody (STJ114363) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.

Anti-SHMT1 antibody (285-444) (STJ114363)

⇓ Datasheet ⇓ SDS
SPECIFICATIONS
ClonalityPolyclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
STJ114363
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General Information

Short DescriptionRabbit polyclonal SHMT1 (285-444) antibody for use in WB, IHC-P, IF, ICC and ELISA in human, mouse and rat samples. Datasheet included with dilution recommendations, and related reagents.
ApplicationsWB/IHC-P/IF/ICC/ELISA
HostRabbit
ReactivityHuman/Mouse/Rat
NoteSTRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.

Product Properties

ClonalityPolyclonal
IsotypeIgG
ConjugationUnconjugated
ConcentrationLot specific
PurificationAffinity purification
Dilution RangeWB:1:500-1:1000
IHC-P:1:50-1:200
IF/ICC:1:50-1:200
ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements.
FormulationPBS with 0.05% Proclin300, 50% Glycerol, pH7.3.
Storage InstructionStore at-20ยฐC for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.

Target Information

Gene SymbolSHMT1
Gene ID6470
Uniprot IDGLYC_HUMAN
Immunogen Region285-444
Immunogen SequenceFKVYQHQVVANCRALSEALT ELGYKIVTGGSDNHLILVDL RSKGTDGGRAEKVLEACSIA CNKNTCPGDRSALRPSGLRL GTPALTSRGLLEKDFQKVAH FIHRGIELTLQIQSDTGVRA TLKEFKERLAGDKYQAAVQA LREEVESFASLFPLPGLPDF
SpecificityRecombinant fusion protein containing a sequence corresponding to amino acids 285-444 of human SHMT1 (NP_683718.1).

Additional Info

Function Interconversion of serine and glycine.
Protein Name Serine Hydroxymethyltransferase - Cytosolic
Shmt
Glycine Hydroxymethyltransferase
Serine Methylase
Database Links Reactome: R-HSA-196757
Reactome: R-HSA-71262
Cellular Localisation Cytoplasm
Alternative Antibody Names Anti-Serine Hydroxymethyltransferase - Cytosolic antibody
Anti-Shmt antibody
Anti-Glycine Hydroxymethyltransferase antibody
Anti-Serine Methylase antibody
Anti-SHMT1 antibody

Information sourced from Uniprot.org

Citations

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