• STJ73671 (2µg/ml) staining of Human Liver lysate (35µg protein in RIPA buffer). Detected by chemiluminescence.
  • STJ73671 Immunofluorescence analysis of paraformaldehyde fixed HeLa cells, permeabilized with 0. 15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml) , showing membrane staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml).
  • STJ73671 Immunofluorescence analysis of paraformaldehyde fixed NIH3T3 cells, permeabilized with 0. 15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml) , showing membrane staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml).
  • STJ73671 Immunofluorescence analysis of paraformaldehyde fixed U251 cells, permeabilized with 0. 15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml) , showing membrane staining. The nuclear stain is DAPI (blue). NA NA NA Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml).
  • STJ73671 Flow cytometric analysis of paraformaldehyde fixed HeLa cells (blue line) , permeabilized with 0. 5% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (1ug/ml). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.

Anti-SHH antibody (C-Term) (STJ73671)

SKU:
STJ73671-100

£303.50
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Host: Goat
Applications: Pep-ELISA/WB/IF/FC
Reactivity: Human/Rat/Dog
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Goat polyclonal antibody anti-SHH (C-Term) is suitable for use in ELISA, Western Blot, Immunofluorescence and Flow Cytometry research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. NA
Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Concentration: 0.5 mg/mL
Dilution Range: WB-1-3µg/ml
IF-Strong expression of the protein seen in the membranes of HeLa, U251 and NIH3T3 cells. 10µg/ml
ELISA-antibody detection limit dilution 1:32000.
Storage Instruction: Store at-20°C on receipt and minimise freeze-thaw cycles.
Gene Symbol: SHH
Gene ID: 6469
Uniprot ID: SHH_HUMAN
Immunogen Region: C-Term
Accession Number: NP_000184.1
Immunogen Sequence: DSEALHPLGMAVK
Function Sonic hedgehog protein: The C-terminal part of the sonic hedgehog protein precursor displays an autoproteolysis and a cholesterol transferase activity. Both activities result in the cleavage of the full-length protein into two parts (ShhN and ShhC) followed by the covalent attachment of a cholesterol moiety to the C-terminal of the newly generated ShhN. Both activities occur in the reticulum endoplasmic. Once cleaved, ShhC is degraded in the endoplasmic reticulum. Sonic hedgehog protein N-product: The dually lipidated sonic hedgehog protein N-product (ShhNp) is a morphogen which is essential for a variety of patterning events during development. Induces ventral cell fate in the neural tube and somites. Involved in the patterning of the anterior-posterior axis of the developing limb bud. Essential for axon guidance. Binds to the patched (PTCH1) receptor, which functions in association with smoothened (SMO), to activate the transcription of target genes. In the absence of SHH, PTCH1 represses the constitutive signaling activity of SMO.
Protein Name Sonic Hedgehog Protein
Shh
Hhg-1
Shh Unprocessed N-Terminal Signaling And C-Terminal Autoprocessing Domains
Shhnc Cleaved Into - Sonic Hedgehog Protein N-Product
Shhn
Shh N-Terminal Processed Signaling Domains
Shhnp
Database Links Reactome: R-HSA-373080
Reactome: R-HSA-5358346
Reactome: R-HSA-5362768
Reactome: R-HSA-5362798
Reactome: R-HSA-5632681
Reactome: R-HSA-5632684
Reactome: R-HSA-5635838
Reactome: R-HSA-5658034
Reactome: R-HSA-9758920
Reactome: R-HSA-9796292
Cellular Localisation Sonic Hedgehog Protein: Endoplasmic Reticulum Membrane
Golgi Apparatus Membrane
Secreted
Co-Localizes With Hhat In The Er And Golgi Membrane
Sonic Hedgehog Protein N-Product: Cell Membrane
Lipid-Anchor
The Dual-Lipidated Sonic Hedgehog Protein N-Product (Shhnp) Is Firmly Tethered To The Cell Membrane Where It Forms Multimers
Further Solubilization And Release From The Cell Surface Seem To Be Achieved Through Different Mechanisms
Including The Interaction With Disp1 And Scube2
Movement By Lipoprotein Particles
Transport By Cellular Extensions Called Cytonemes Or By The Proteolytic Removal Of Both Terminal Lipidated Peptides
Alternative Antibody Names Anti-Sonic Hedgehog Protein antibody
Anti-Shh antibody
Anti-Hhg-1 antibody
Anti-Shh Unprocessed N-Terminal Signaling And C-Terminal Autoprocessing Domains antibody
Anti-Shhnc Cleaved Into - Sonic Hedgehog Protein N-Product antibody
Anti-Shhn antibody
Anti-Shh N-Terminal Processed Signaling Domains antibody
Anti-Shhnp antibody
Anti-SHH antibody

Information sourced from Uniprot.org

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