Anti-SHH antibody (187-281) (STJ11102952)
SPECIFICATIONS
ClonalityPolyclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
General Information
| Short Description | Rabbit polyclonal SHH (187-281) antibody for use in WB and ELISA in human, mouse and rat samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications | WB/ELISA |
| Host | Rabbit |
| Reactivity | Human/Mouse/Rat |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Concentration | Lot specific |
| Purification | Affinity purification |
| Dilution Range | WB:1:500-1:1000ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements. |
| Formulation | PBS with 0.01% Thimerosal, 50% Glycerol, pH 7.3. |
| Storage Instruction | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
Target Information
| Gene Symbol | Shh |
| Gene ID | 20423 |
| Uniprot ID | SHH_MOUSE |
| Immunogen Region | 187-281 |
| Immunogen Sequence | KAENSVAAKSGGCFPGSATV HLEQGGTKLVKDLRPGDRVL AADDQGRLLYSDFLTFLDRD EGAKKVFYVIETLEPRERLL LTAAHLLFVAPHNDS |
| Specificity | Recombinant fusion protein containing a sequence corresponding to amino acids 187-281 of mouse SHH (NP_033196.1). |
Additional Info
| Tissue Specificity | Expressed in a number of embryonic tissues including the notochord, ventral neural tube, floor plate, lung bud, zone of polarizing activity and posterior distal mesenchyme of limbs. In the adult, expressed in lung and neural retina. |
| Post Translational Modifications | Sonic hedgehog protein: The C-terminal domain displays an autoproteolysis activity and a cholesterol transferase activity. Both activities result in the cleavage of the full-length protein and covalent attachment of a cholesterol moiety to the C-terminal of the newly generated N-terminal fragment (ShhN). Cholesterylation is required for the sonic hedgehog protein N-product targeting to lipid rafts and multimerization. ShhN is the active species in both local and long-range signaling, whereas the C-product (ShhC) is degraded in the endoplasmic reticulum. Sonic hedgehog protein N-product: N-palmitoylation by HHAT of ShhN is required for sonic hedgehog protein N-product multimerization and full activity. It is a prerequisite for the membrane-proximal positioning and the subsequent shedding of this N-terminal peptide. Sonic hedgehog protein N-product: The lipidated N- and C-terminal peptides of ShhNp can be cleaved (shedding). The N-terminal palmitoylated peptide is cleaved at the Cardin-Weintraub (CW) motif site. The cleavage reduced the interactions with heparan sulfate. The cleavage is enhanced by SCUBE2. |
| Function | Sonic hedgehog protein: The C-terminal part of the sonic hedgehog protein precursor displays an autoproteolysis and a cholesterol transferase activity. Both activities result in the cleavage of the full-length protein into two parts (ShhN and ShhC) followed by the covalent attachment of a cholesterol moiety to the C-terminal of the newly generated ShhN. Both activities occur in the reticulum endoplasmic. Once cleaved, ShhC is degraded in the endoplasmic reticulum. Sonic hedgehog protein N-product: The dually lipidated sonic hedgehog protein N-product (ShhNp) is a morphogen which is essential for a variety of patterning events during development. Induces ventral cell fate in the neural tube and somites. Involved in the patterning of the anterior-posterior axis of the developing limb bud. Essential for axon guidance. Binds to the patched (PTCH1) receptor, which functions in association with smoothened (SMO), to activate the transcription of target genes. In the absence of SHH, PTCH1 represses the constitutive signaling activity of SMO. |
| Protein Name | Sonic Hedgehog ProteinShhHhg-1Shh Unprocessed N-Terminal Signaling And C-Terminal Autoprocessing DomainsShhnc Cleaved Into - Sonic Hedgehog Protein N-ProductShhnShh N-Terminal Processed Signaling DomainsShhnpSonic Hedgehog Protein 19 Kda Product |
| Database Links | Reactome: R-MMU-5358346Reactome: -MMU-5362798Reactome: -MMU-5632681Reactome: -MMU-5635838 |
| Cellular Localisation | Sonic Hedgehog Protein: Endoplasmic Reticulum MembraneGolgi Apparatus MembraneCo-Localizes With Hhat In The Er And Golgi MembraneSonic Hedgehog Protein N-Product: Cell MembraneLipid-AnchorThe Dual-Lipidated Sonic Hedgehog Protein N-Product (Shhnp) Is Firmly Tethered To The Cell Membrane Where It Forms MultimersFurther Solubilization And Release From The Cell Surface Seem To Be Achieved Through Different MechanismsIncluding The Interaction With Disp1 And Scube2Movement By Lipoprotein ParticlesTransport By Cellular Extensions Called Cytonemes Or By The Proteolytic Removal Of Both Terminal Lipidated Peptides |
| Alternative Antibody Names | Anti-Sonic Hedgehog Protein antibodyAnti-Shh antibodyAnti-Hhg-1 antibodyAnti-Shh Unprocessed N-Terminal Signaling And C-Terminal Autoprocessing Domains antibodyAnti-Shhnc Cleaved Into - Sonic Hedgehog Protein N-Product antibodyAnti-Shhn antibodyAnti-Shh N-Terminal Processed Signaling Domains antibodyAnti-Shhnp antibodyAnti-Sonic Hedgehog Protein 19 Kda Product antibodyAnti-Shh antibodyAnti-Hhg1 antibody |
Information sourced from Uniprot.org