| Host: |
NZ White Rabbit |
| Applications: |
IHC/WB |
| Reactivity: |
Human/Rat/Mouse |
| Note: |
STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description: |
Nz White Rabbit polyclonal antibody anti-SHANK3 is suitable for use in Immunohistochemistry and Western Blot research applications. |
| Clonality: |
Polyclonal |
| Conjugation: |
Unconjugated |
| Isotype: |
IgG |
| Formulation: |
Shipped as lyophilised. Reconstitute in 500 µl of sterile water. Centrifuge to remove any insoluble material. |
| Purification: |
Ammonium sulphate precipitation |
| Dilution Range: |
A concentration of 10-50 µg/ml is recommended. The optimal concentration should be determined by the end user. Not yet tested in other applications. |
| Storage Instruction: |
Maintain the lyophilised/reconstituted antibodies frozen at-20°C for long term storage and refrigerated at 2-8°C for a shorter term. When reconstituting, glycerol (1:1) may be added for an additional stability. Avoid freeze and thaw cycles. |
| Gene Symbol: |
SHANK3Uniprot ID=SHAN3_HUMAN" |
| Specificity: |
Specific for SHANK3. |
| Immunogen: |
A synthetic peptide from human SHANK3 conjugated to blue carrier protein was used as the antigen. |
| Function | Major scaffold postsynaptic density protein which interacts with multiple proteins and complexes to orchestrate the dendritic spine and synapse formation, maturation and maintenance. Interconnects receptors of the postsynaptic membrane including NMDA-type and metabotropic glutamate receptors via complexes with GKAP/PSD-95 and HOMER, respectively, and the actin-based cytoskeleton. Plays a role in the structural and functional organization of the dendritic spine and synaptic junction through the interaction with Arp2/3 and WAVE1 complex as well as the promotion of the F-actin clusters. By way of this control of actin dynamics, participates in the regulation of developing neurons growth cone motility and the NMDA receptor-signaling. Also modulates GRIA1 exocytosis and GRM5/MGLUR5 expression and signaling to control the AMPA and metabotropic glutamate receptor-mediated synaptic transmission and plasticity. May be required at an early stage of synapse formation and be inhibited by IGF1 to promote synapse maturation. |
| Protein Name | Sh3 And Multiple Ankyrin Repeat Domains Protein 3Shank3Proline-Rich Synapse-Associated Protein 2Prosap2 |
| Cellular Localisation | CytoplasmPostsynaptic DensityCell ProjectionDendritic SpineIn Neuronal CellsExtends Into The Region Subjacent To The Postsynaptic Density (Psd) |
| Alternative Antibody Names | Anti-Sh3 And Multiple Ankyrin Repeat Domains Protein 3 antibodyAnti-Shank3 antibodyAnti-Proline-Rich Synapse-Associated Protein 2 antibodyAnti-Prosap2 antibodyAnti-SHANK3 antibodyAnti-KIAA1650 antibodyAnti-PROSAP2 antibodyAnti-PSAP2 antibody |
Information sourced from Uniprot.org
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
