• Immunohistochemistry analysis of Bif-1 in paraffin-embedded Rat testis using Bif-1 Rabbit polyclonal antibody (STJ117087) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Western blot analysis of various lysates using Bif-1 Rabbit polyclonal antibody (STJ117087) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 60s.

Anti-SH3GLB1 antibody (120-365) (STJ117087)

SKU:
STJ117087

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Host: Rabbit
Applications: WB/IHC
Reactivity: Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-Bif-1 (120-365) is suitable for use in Western Blot and Immunohistochemistry research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.01% Thimerosal, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
IHC-P 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: SH3GLB1
Gene ID: 51100
Uniprot ID: SHLB1_HUMAN
Immunogen Region: 120-365
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 120-365 of human Bif-1 (NP_057093.1).
Immunogen Sequence: KCGETQKRIGTADRELIQTS ALNFLTPLRNFIEGDYKTIA KERKLLQNKRLDLDAAKTRL KKAKAAETRNSSEQELRITQ SEFDRQAEITRLLLEGISST HAHHLRCLNDFVEAQMTYYA QCYQYMLDLQKQLGSFPSNY LSNNNQTSVTPVPSVLPNAI GSSAMASTSGLVITSPSNLS DLKECSGSRKARVLYDYDAA NSTELSLLADEVITVFSVVG MDSDWLMGERGNQKGKVPI
Tissue Specificity Highly expressed in heart, skeletal muscle, kidney and placenta. Detected at lower levels in brain, colon, thymus, spleen, liver, small intestine, lung and peripheral blood leukocytes.
Post Translational Modifications Phosphorylated at Thr-145 by CDK5.this phosphorylation is required for autophagy induction in starved neurons and facilitates homodimerization.
Function May be required for normal outer mitochondrial membrane dynamics. Required for coatomer-mediated retrograde transport in certain cells. May recruit other proteins to membranes with high curvature. May promote membrane fusion. Involved in activation of caspase-dependent apoptosis by promoting BAX/BAK1 activation. Isoform 1 acts proapoptotic in fibroblasts. Involved in caspase-independent apoptosis during nutrition starvation and involved in the regulation of autophagy. Activates lipid kinase activity of PIK3C3 during autophagy probably by associating with the PI3K complex II (PI3KC3-C2). Associated with PI3KC3-C2 during autophagy may regulate the trafficking of ATG9A from the Golgi complex to the peripheral cytoplasm for the formation of autophagosomes by inducing Golgi membrane tubulation and fragmentation. Involved in regulation of degradative endocytic trafficking and cytokinesis, probably in the context of PI3KC3-C2. Isoform 2 acts antiapoptotic in neuronal cells.involved in maintenance of mitochondrial morphology and promotes neuronal viability.
Protein Name Endophilin-B1
Bax-Interacting Factor 1
Bif-1
Sh3 Domain-Containing Grb2-Like Protein B1
Cellular Localisation Cytoplasm
Golgi Apparatus Membrane
Peripheral Membrane Protein
Mitochondrion Outer Membrane
Cytoplasmic Vesicle
Autophagosome Membrane
Midbody
Association With The Golgi Apparatus Depends On The Cell Type
Following Starvation Colocalizes With Atg5 And Lc3 Autophagy-Related Protein(S)On Autophagosomal Membranes
Alternative Antibody Names Anti-Endophilin-B1 antibody
Anti-Bax-Interacting Factor 1 antibody
Anti-Bif-1 antibody
Anti-Sh3 Domain-Containing Grb2-Like Protein B1 antibody
Anti-SH3GLB1 antibody
Anti-KIAA0491 antibody
Anti-CGI-61 antibody

Information sourced from Uniprot.org

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