Anti-SH3GLB1 antibody (1-365) (STJ29184)
SPECIFICATIONS
ClonalityPolyclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
General Information
| Short Description | Rabbit polyclonal Bif-1 (1-365) antibody for use in IF, ICC and ELISA in human samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications | IF/ICC/ELISA |
| Host | Rabbit |
| Reactivity | Human |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Concentration | Lot specific |
| Purification | Affinity purification |
| Dilution Range | IF/ICC:1:50-1:100ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements. |
| Formulation | PBS with 0.02% Sodium Azide, 50% Glycerol, pH 7.3. |
| Storage Instruction | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
Target Information
| Gene Symbol | SH3GLB1 |
| Gene ID | 51100 |
| Uniprot ID | SHLB1_HUMAN |
| Immunogen Region | 1-365 |
| Immunogen Sequence | MNIMDFNVKKLAADAGTFLS RAVQFTEEKLGQAEKTELDA HLENLLSKAECTKIWTEKIM KQTEVLLQPNPNARIEEFVY EKLDRKAPSRINNPELLGQY MIDAGTEFGPGTAYGNALIK CGETQKRIGTADRELIQTSA LNFLTPLRNFIEGDYKTIAK ERKLLQNKRLDLDAAKTRLK KAKAAETRNSSEQELRITQS EFDRQAEITRLLLEGISSTH AHHLRCLNDFVEAQMTYYA |
| Specificity | Recombinant fusion protein containing a sequence corresponding to amino acids 1-365 of human Bif-1 (NP_057093.1). |
Additional Info
| Tissue Specificity | Highly expressed in heart, skeletal muscle, kidney and placenta. Detected at lower levels in brain, colon, thymus, spleen, liver, small intestine, lung and peripheral blood leukocytes. |
| Post Translational Modifications | Phosphorylated at Thr-145 by CDK5.this phosphorylation is required for autophagy induction in starved neurons and facilitates homodimerization. |
| Function | May be required for normal outer mitochondrial membrane dynamics. Required for coatomer-mediated retrograde transport in certain cells. May recruit other proteins to membranes with high curvature. May promote membrane fusion. Involved in activation of caspase-dependent apoptosis by promoting BAX/BAK1 activation. Isoform 1 acts proapoptotic in fibroblasts. Involved in caspase-independent apoptosis during nutrition starvation and involved in the regulation of autophagy. Activates lipid kinase activity of PIK3C3 during autophagy probably by associating with the PI3K complex II (PI3KC3-C2). Associated with PI3KC3-C2 during autophagy may regulate the trafficking of ATG9A from the Golgi complex to the peripheral cytoplasm for the formation of autophagosomes by inducing Golgi membrane tubulation and fragmentation. Involved in regulation of degradative endocytic trafficking and cytokinesis, probably in the context of PI3KC3-C2. Isoform 2 acts antiapoptotic in neuronal cells.involved in maintenance of mitochondrial morphology and promotes neuronal viability. |
| Protein Name | Endophilin-B1Bax-Interacting Factor 1Bif-1Sh3 Domain-Containing Grb2-Like Protein B1 |
| Database Links | |
| Cellular Localisation | CytoplasmGolgi Apparatus MembranePeripheral Membrane ProteinMitochondrion Outer MembraneCytoplasmic VesicleAutophagosome MembraneMidbodyAssociation With The Golgi Apparatus Depends On The Cell TypeFollowing Starvation Colocalizes With Atg5 And Lc3 Autophagy-Related Protein(S)On Autophagosomal Membranes |
| Alternative Antibody Names | Anti-Endophilin-B1 antibodyAnti-Bax-Interacting Factor 1 antibodyAnti-Bif-1 antibodyAnti-Sh3 Domain-Containing Grb2-Like Protein B1 antibodyAnti-SH3GLB1 antibodyAnti-KIAA0491 antibodyAnti-CGI-61 antibody |
Information sourced from Uniprot.org