• Immunofluorescence analysis of HeLa cells using SH3GL2 antibody (STJ27668). Blue: DAPI for nuclear staining.
  • Western blot analysis of extracts of various cell lines, using SH3GL2 antibody (STJ27668) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.

Anti-SH3GL2 antibody (1-270) (STJ27668)

SKU:
STJ27668

£169.50 - £449.50
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Host: Rabbit
Applications: WB/IF
Reactivity: Human/Mouse
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-SH3GL2 (1-270) is suitable for use in Western Blot and Immunofluorescence research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
IF/ICC 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: SH3GL2
Gene ID: 6456
Uniprot ID: SH3G2_HUMAN
Immunogen Region: 1-270
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-270 of human SH3GL2 (NP_003017.1).
Immunogen Sequence: MSVAGLKKQFHKATQKVSEK VGGAEGTKLDDDFKEMERKV DVTSRAVMEIMTKTIEYLQP NPASRAKLSMINTMSKIRGQ EKGPGYPQAEALLAEAMLKF GRELGDDCNFGPALGEVGEA MRELSEVKDSLDIEVKQNFI DPLQNLHDKDLREIQHHLKK LEGRRLDFDYKKKRQGKIPD EELRQALEKFDESKEIAESS MFNLLEMDIEQVSQLSALVQ AQLEYHKQAVQILQQVTVR
Tissue Specificity Brain, mostly in frontal cortex. Expressed at high level in fetal cerebellum.
Function Implicated in synaptic vesicle endocytosis. May recruit other proteins to membranes with high curvature. Required for BDNF-dependent dendrite outgrowth. Cooperates with SH3GL2 to mediate BDNF-NTRK2 early endocytic trafficking and signaling from early endosomes.
Protein Name Endophilin-A1
Een-B1
Endophilin-1
Sh3 Domain Protein 2a
Sh3 Domain-Containing Grb2-Like Protein 2
Database Links Reactome: R-HSA-177504
Reactome: R-HSA-182971
Reactome: R-HSA-2132295
Reactome: R-HSA-432720
Reactome: R-HSA-432722
Reactome: R-HSA-437239
Reactome: R-HSA-6807004
Reactome: R-HSA-8856825
Reactome: R-HSA-8856828
Reactome: R-HSA-8875360
Cellular Localisation Cytoplasm
Membrane
Peripheral Membrane Protein
Early Endosome
Presynapse
Alternative Antibody Names Anti-Endophilin-A1 antibody
Anti-Een-B1 antibody
Anti-Endophilin-1 antibody
Anti-Sh3 Domain Protein 2a antibody
Anti-Sh3 Domain-Containing Grb2-Like Protein 2 antibody
Anti-SH3GL2 antibody
Anti-CNSA2 antibody
Anti-SH3D2A antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance