• Immunofluorescence analysis of U-2 OS cells using SH3BGRL Rabbit polyclonal antibody (STJ119622) at dilution of 1:100. Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.
  • Western blot analysis of various lysates using SH3BGRL Rabbit polyclonal antibody (STJ119622) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.

Anti-SH3BGRL antibody (1-114) (STJ119622)

SKU:
STJ119622

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Host: Rabbit
Applications: WB/IF
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-SH3BGRL (1-114) is suitable for use in Western Blot and Immunofluorescence research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.01% Thimerosal, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
IF/ICC 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: SH3BGRL
Gene ID: 6451
Uniprot ID: SH3L1_HUMAN
Immunogen Region: 1-114
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-114 of human SH3BGRL (NP_003013.1).
Immunogen Sequence: MVIRVYIASSSGSTAIKKKQ QDVLGFLEANKIGFEEKDIA ANEENRKWMRENVPENSRPA TGYPLPPQIFNESQYRGDYD AFFEARENNAVYAFLGLTAP PGSKEAEVQAKQQA
Tissue Specificity Ubiquitous.
Function Appears to function as an adapter protein that bridges proteins together or proteins with mRNAs. May function as a ubiquitin ligase-substrate adapter. Additionally, associates with translating cytoplasmic ribosomes and may promote the expression of specific mRNAs.
Protein Name Adapter Sh3bgrl
Sh3 Domain-Binding Glutamic Acid-Rich-Like Protein 1
Cellular Localisation Cytoplasm
Cytosol
Cell Membrane
Alternative Antibody Names Anti-Adapter Sh3bgrl antibody
Anti-Sh3 Domain-Binding Glutamic Acid-Rich-Like Protein 1 antibody
Anti-SH3BGRL antibody

Information sourced from Uniprot.org

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