| Host: |
Rabbit |
| Applications: |
WB/IF |
| Reactivity: |
Human/Mouse |
| Note: |
STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description: |
Rabbit polyclonal antibody anti-SH2D1A (1-128) is suitable for use in Western Blot and Immunofluorescence research applications. |
| Clonality: |
Polyclonal |
| Conjugation: |
Unconjugated |
| Isotype: |
IgG |
| Formulation: |
PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3. |
| Purification: |
Affinity purification |
| Dilution Range: |
WB 1:500-1:2000IF/ICC 1:50-1:200 |
| Storage Instruction: |
Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: |
SH2D1A |
| Gene ID: |
4068 |
| Uniprot ID: |
SH21A_HUMAN |
| Immunogen Region: |
1-128 |
| Immunogen: |
Recombinant fusion protein containing a sequence corresponding to amino acids 1-128 of human SH2D1A (NP_002342.1). |
| Immunogen Sequence: |
MDAVAVYHGKISRETGEKLL LATGLDGSYLLRDSESVPGV YCLCVLYHGYIYTYRVSQTE TGSWSAETAPGVHKRYFRKI KNLISAFQKPDQGIVIPLQY PVEKKSSARSTQGTTGIRED PDVCLKAP |
| Tissue Specificity | Expressed at a high level in thymus and lung, with a lower level of expression in spleen and liver. Expressed in peripheral blood leukocytes, including T-lymphocytes. Tends to be expressed at lower levels in peripheral blood leukocytes in patients with rheumatoid arthritis. |
| Function | Cytoplasmic adapter regulating receptors of the signaling lymphocytic activation molecule (SLAM) family such as SLAMF1, CD244, LY9, CD84, SLAMF6 and SLAMF7. In SLAM signaling seems to cooperate with SH2D1B/EAT-2. Initially it has been proposed that association with SLAMF1 prevents SLAMF1 binding to inhibitory effectors including INPP5D/SHIP1 and PTPN11/SHP-2. However, by simultaneous interactions, recruits FYN which subsequently phosphorylates and activates SLAMF1. Positively regulates CD244/2B4- and CD84-mediated natural killer (NK) cell functions. Can also promote CD48-, SLAMF6 -, LY9-, and SLAMF7-mediated NK cell activation. In the context of NK cell-mediated cytotoxicity enhances conjugate formation with target cells. May also regulate the activity of the neurotrophin receptors NTRK1, NTRK2 and NTRK3. |
| Protein Name | Sh2 Domain-Containing Protein 1aDuncan Disease Sh2-ProteinSignaling Lymphocytic Activation Molecule-Associated ProteinSlam-Associated ProteinT-Cell Signal Transduction Molecule Sap |
| Database Links | Reactome: R-HSA-198933 |
| Cellular Localisation | Cytoplasm |
| Alternative Antibody Names | Anti-Sh2 Domain-Containing Protein 1a antibodyAnti-Duncan Disease Sh2-Protein antibodyAnti-Signaling Lymphocytic Activation Molecule-Associated Protein antibodyAnti-Slam-Associated Protein antibodyAnti-T-Cell Signal Transduction Molecule Sap antibodyAnti-SH2D1A antibodyAnti-DSHP antibodyAnti-SAP antibody |
Information sourced from Uniprot.org
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
![Western blot analysis of lysates from wild type (WT) and Histone H2A. Z knockout (KO) 293T cells, using [KO Validated] Histone H2A. Z Rabbit polyclonal antibody (STJ114316) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.](https://cdn11.bigcommerce.com/s-zso2xnchw9/images/stencil/300x300/products/93438/374520/STJ114316_1__91357.1713141034.jpg?c=1 "Western blot analysis of lysates from wild type (WT) and Histone H2A. Z knockout (KO) 293T cells, using [KO Validated] Histone H2A. Z Rabbit polyclonal antibody (STJ114316) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.")