| Host: |
Rabbit |
| Applications: |
IHC/IF |
| Reactivity: |
Human/Mouse/Rat |
| Note: |
STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description: |
Rabbit polyclonal antibody anti-SH2B1 (360-520) is suitable for use in Immunohistochemistry and Immunofluorescence research applications. |
| Clonality: |
Polyclonal |
| Conjugation: |
Unconjugated |
| Isotype: |
IgG |
| Formulation: |
PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3. |
| Purification: |
Affinity purification |
| Dilution Range: |
IHC-P 1:50-1:200IF/ICC 1:20-1:50 |
| Storage Instruction: |
Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: |
SH2B1 |
| Gene ID: |
25970 |
| Uniprot ID: |
SH2B1_HUMAN |
| Immunogen Region: |
360-520 |
| Immunogen: |
Recombinant fusion protein containing a sequence corresponding to amino acids 360-520 of human SH2B1 (NP_056318.2). |
| Immunogen Sequence: |
DAQHVKAWVSDIQECLSPGP CPATSPRPMTLPLAPGTSFL TRENTDSLELSCLNHSESLP SQDLLLGPSESNDRLSQGAY GGLSDRPSASISPSSASIAA SHFDSMELLPPELPPRIPIE EGPPTGTVHPLSAPYPPLDT PETATGSFLFQGEPEGGEGD Q |
| Tissue Specificity | Widely expressed with highest levels in skeletal muscle and ovary. |
| Post Translational Modifications | Phosphorylated on tyrosine residues in response to receptor kinase stimulation. Phosphorylated by RET. |
| Function | Adapter protein for several members of the tyrosine kinase receptor family. Involved in multiple signaling pathways mediated by Janus kinase (JAK) and receptor tyrosine kinases, including the receptors of insulin (INS), insulin-like growth factor I (IGF1), nerve growth factor (NGF), brain-derived neurotrophic factor (BDNF), glial cell line-derived neurotrophic factor (GDNF), platelet-derived growth factor (PDGF) and fibroblast growth factors (FGFs). In growth hormone (GH) signaling, autophosphorylated ('Tyr-813') JAK2 recruits SH2B1, which in turn is phosphorylated by JAK2 on tyrosine residues. These phosphotyrosines form potential binding sites for other signaling proteins. GH also promotes serine/threonine phosphorylation of SH2B1 and these phosphorylated residues may serve to recruit other proteins to the GHR-JAK2-SH2B1 complexes, such as RAC1. In leptin (LEP) signaling, binds to and potentiates the activation of JAK2 by globally enhancing downstream pathways. In response to leptin, binds simultaneously to both, JAK2 and IRS1 or IRS2, thus mediating formation of a complex of JAK2, SH2B1 and IRS1 or IRS2. Mediates tyrosine phosphorylation of IRS1 and IRS2, resulting in activation of the PI 3-kinase pathway. Acts as positive regulator of NGF-mediated activation of the Akt/Forkhead pathway.prolongs NGF-induced phosphorylation of AKT1 on 'Ser-473' and AKT1 enzymatic activity. Enhances the kinase activity of the cytokine receptor-associated tyrosine kinase JAK2 and of other receptor tyrosine kinases, such as FGFR3 and NTRK1. For JAK2, the mechanism seems to involve dimerization of both, SH2B1 and JAK2. Enhances RET phosphorylation and kinase activity. Isoforms seem to be differentially involved in IGF-I and PDGF-induced mitogenesis. |
| Protein Name | Sh2b Adapter Protein 1Pro-Rich - Ph And Sh2 Domain-Containing Signaling MediatorPsmSh2 Domain-Containing Protein 1b |
| Database Links | Reactome: R-HSA-1170546 Q9NRF2-2Reactome: R-HSA-2586552 Q9NRF2-2Reactome: R-HSA-982772 Q9NRF2-2Reactome: R-HSA-983231 |
| Cellular Localisation | CytoplasmMembraneNucleusShuttles Between The Nucleus And The Cytoplasm |
| Alternative Antibody Names | Anti-Sh2b Adapter Protein 1 antibodyAnti-Pro-Rich - Ph And Sh2 Domain-Containing Signaling Mediator antibodyAnti-Psm antibodyAnti-Sh2 Domain-Containing Protein 1b antibodyAnti-SH2B1 antibodyAnti-KIAA1299 antibodyAnti-SH2B antibody |
Information sourced from Uniprot.org
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
