Anti-SGO1 antibody (271-320 aa) (STJ95640)
SPECIFICATIONS
ClonalityPolyclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
ImmunogenThe antiserum was produced against synthesized peptide derived from the human SGOL1 at the amino acid range 271-320
General Information
| Short Description | Rabbit polyclonal anti-Shugoshin 1 (271-320 aa) for use in WB, IHC, IF and ELISA in Human, Rat and Mouse samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications | WB/IHC/IF/ELISA |
| Host | Rabbit |
| Reactivity | Human/Rat/Mouse |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Concentration | 1 mg/mL |
| Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Dilution Range | WB 1:500-1:2000IHC 1:100-1:300ELISA 1:20000IF 1:50-200 |
| Formulation | Liquid in PBS containing 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide. |
| Storage Instruction | Store at-20ยฐC for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
Target Information
| Gene Symbol | SGO1 |
| Gene ID | 151648 |
| Uniprot ID | SGO1_HUMAN |
| Immunogen | The antiserum was produced against synthesized peptide derived from the human SGOL1 at the amino acid range 271-320 |
| Immunogen Region | 271-320 aa |
| Specificity | Sgo1 Polyclonal Antibody detects endogenous levels of Sgo1 protein. |
Additional Info
| Post Translational Modifications | Ubiquitinated and degraded during mitotic exit by APC/C-Cdh1. Phosphorylation by NEK2 is essential for chromosome congression in mitosis and for the proper attachment of spindle microtubule to the kinetochore. Phosphorylated by PLK1 and AUKRB. |
| Function | Plays a central role in chromosome cohesion during mitosis by preventing premature dissociation of cohesin complex from centromeres after prophase, when most of cohesin complex dissociates from chromosomes arms. May act by preventing phosphorylation of the STAG2 subunit of cohesin complex at the centromere, ensuring cohesin persistence at centromere until cohesin cleavage by ESPL1/separase at anaphase. Essential for proper chromosome segregation during mitosis and this function requires interaction with PPP2R1A. Its phosphorylated form is necessary for chromosome congression and for the proper attachment of spindle microtubule to the kinetochore. Necessary for kinetochore localization of PLK1 and CENPF. May play a role in the tension sensing mechanism of the spindle-assembly checkpoint by regulating PLK1 kinetochore affinity. Isoform 3 plays a role in maintaining centriole cohesion involved in controlling spindle pole integrity. Involved in centromeric enrichment of AUKRB in prometaphase. |
| Protein Name | Shugoshin 1Serologically Defined Breast Cancer Antigen Ny-Br-85Shugoshin-Like 1 |
| Database Links | Reactome: R-HSA-141444Reactome: R-HSA-2467813Reactome: R-HSA-2500257Reactome: R-HSA-5663220Reactome: R-HSA-68877Reactome: R-HSA-9648025 |
| Cellular Localisation | NucleusChromosomeCentromereKinetochoreCytoplasmCytoskeletonSpindle PoleMicrotubule Organizing CenterCentrosomeNucleus SpeckleLocalizes To The Inner Centromere Throughout Prophase Until Metaphase And Disappears At AnaphaseCentromeric Localization Requires The Presence Of Bub1 And The Interaction With Ppp2r1aColocalizes With Nek2 At The KinetochoreColocalizes With And Ss18l1 At The KinetochorePhosphorylation By Aukrb And The Presence Of Bub1 Are Required For Localization To The KinetochoreIsoform 1 Primarily Localizes To Kinetochores During G2 Phase And Mitotic ProphaseMetaphaseAnd Anaphase And Does Not Appear To Be Associated With Kinetochores During Late MitosisIsoform 3 Is Found At The Centrosome In Interphase And At Spindle Poles In Mitosis And Its Spindle Pole Localization Is Plk1 DependentIsoform 3 Does Not Localize To Kinetochores During Any Stages Of The Cell Cycle |
| Alternative Antibody Names | Anti-Shugoshin 1 antibodyAnti-Serologically Defined Breast Cancer Antigen Ny-Br-85 antibodyAnti-Shugoshin-Like 1 antibodyAnti-SGO1 antibodyAnti-SGOL1 antibody |
Information sourced from Uniprot.org