• Immunofluorescence analysis of mouse pancreas using SFRP5 Polyclonal Antibody (STJ119145) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
  • Western blot analysis of various lysates, using SFRP5 antibody (STJ119145) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 90s.

Anti-SFRP5 antibody (30-317) (STJ119145)

SKU:
STJ119145

€140.63 - €653.13
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Host: Rabbit
Applications: WB/IHC/IF
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-SFRP5 (30-317) is suitable for use in Western Blot, Immunohistochemistry and Immunofluorescence research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.01% Thimerosal, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:1000
IHC-P 1:50-1:100
IF/ICC 1:50-1:100
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: SFRP5
Gene ID: 6425
Uniprot ID: SFRP5_HUMAN
Immunogen Region: 30-317
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 30-317 of human SFRP5 (NP_003006.2).
Immunogen Sequence: EEYDYYGWQAEPLHGRSYSK PPQCLDIPADLPLCHTVGYK RMRLPNLLEHESLAEVKQQA SSWLPLLAKRCHSDTQVFLC SLFAPVCLDRPIYPCRSLCE AVRAGCAPLMEAYGFPWPEM LHCHKFPLDNDLCIAVQFGH LPATAPPVTKICAQCEMEHS ADGLMEQMCSSDFVVKMRIK EIKIENGDRKLIGAQKKKKL LKPGPLKRKDTKRLVLHMKN GAGCPCPQLDSLAGSFLVM
Tissue Specificity Highly expressed in the retinal pigment epithelium (RPE) and pancreas. Weak expression in heart, liver and muscle.
Function Soluble frizzled-related proteins (sFRPS) function as modulators of Wnt signaling through direct interaction with Wnts. They have a role in regulating cell growth and differentiation in specific cell types. SFRP5 may be involved in determining the polarity of photoreceptor, and perhaps, other cells in the retina.
Protein Name Secreted Frizzled-Related Protein 5
Sfrp-5
Frizzled-Related Protein 1b
Frp-1b
Secreted Apoptosis-Related Protein 3
Sarp-3
Cellular Localisation Secreted
Alternative Antibody Names Anti-Secreted Frizzled-Related Protein 5 antibody
Anti-Sfrp-5 antibody
Anti-Frizzled-Related Protein 1b antibody
Anti-Frp-1b antibody
Anti-Secreted Apoptosis-Related Protein 3 antibody
Anti-Sarp-3 antibody
Anti-SFRP5 antibody
Anti-FRP1B antibody
Anti-SARP3 antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance