• Immunoflourescence of HeLa cells labeled with anti-SAP49 (STJA0003775, red, 1:1000) and anti-vimentin (STJA0003823, green, 1:10,000). The blue is DAPI staining nuclear DNA.
  • Immunofluorescence of HeLa cells showing strong granular nuclear staining of SAP49 (red, 1:1000) and vimentin (STJA0003823, green, 1:10,000). The blue is DAPI staining nuclear DNA.
  • Western blot of HeLa lysate showing specific immunolabeling of the ~49 kDa SAP49 protein.

Anti-SF3B4 antibody (Full Length) [3A1] (STJA0003775)

SKU:
STJA0003775-100

$613.58
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Host: Mouse
Applications: WB/IHC/ICC
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Mouse monoclonal antibody anti-SAP49 (Full Length) is suitable for use in Western Blot, Immunohistochemistry and Immunocytochemistry research applications.
Clonality: Monoclonal
Clone ID: 3A1
Conjugation: Unconjugated
Isotype: IgG2b
Formulation: 100 ul in PBS + 50% Glycerol and 5 mm Sodium Azide
Purification: This antibody was protein g purified culture from supernatant.
Dilution Range: WB 1:2000
IHC 1:1000
ICC 1:1000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: SF3B4
Gene ID: 10262
Uniprot ID: SF3B4_HUMAN
Immunogen Region: Full Length
Immunogen: Recombinant full length human SAP49 expressed in and purification from E. Coli.
Function Component of the 17S U2 SnRNP complex of the spliceosome, a large ribonucleoprotein complex that removes introns from transcribed pre-mRNAs. The 17S U2 SnRNP complex (1) directly participates in early spliceosome assembly and (2) mediates recognition of the intron branch site during pre-mRNA splicing by promoting the selection of the pre-mRNA branch-site adenosine, the nucleophile for the first step of splicing. Within the 17S U2 SnRNP complex, SF3B4 is part of the SF3B subcomplex, which is required for 'A' complex assembly formed by the stable binding of U2 snRNP to the branchpoint sequence in pre-mRNA. Sequence independent binding of SF3A and SF3B subcomplexes upstream of the branch site is essential, it may anchor U2 snRNP to the pre-mRNA. May also be involved in the assembly of the 'E' complex. Also acts as a component of the minor spliceosome, which is involved in the splicing of U12-type introns in pre-mRNAs.
Protein Name Splicing Factor 3b Subunit 4
Pre-Mrna-Splicing Factor Sf3b 49 Kda Subunit
Spliceosome-Associated Protein 49
Sap 49
Database Links Reactome: R-HSA-72163
Reactome: R-HSA-72165
Cellular Localisation Nucleus
Alternative Antibody Names Anti-Splicing Factor 3b Subunit 4 antibody
Anti-Pre-Mrna-Splicing Factor Sf3b 49 Kda Subunit antibody
Anti-Spliceosome-Associated Protein 49 antibody
Anti-Sap 49 antibody
Anti-SF3B4 antibody
Anti-SAP49 antibody

Information sourced from Uniprot.org

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