| Short Description : | Rabbit polyclonal SF3A3 (292-501) antibody for use in WB, IHC-P, IF, ICC and ELISA in human, mouse and rat samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications: | WB/IHC-P/IF/ICC/ELISA |
| Host: | Rabbit |
| Reactivity: | Human/Mouse/Rat |
| Note: | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Clonality : | Polyclonal |
| Isotype: | IgG |
| Conjugation: | Unconjugated |
| Concentration: | Lot specific |
| Purification: | Affinity purification |
| Dilution Range: | WB:1:500-1:1000IHC-P:1:50-1:200IF/ICC:1:50-1:200ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements. |
| Formulation: | PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3. |
| Storage Instruction: | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: | SF3A3 |
| Gene ID: | 10946 |
| Uniprot ID: | SF3A3_HUMAN |
| Immunogen Region: | 292-501 |
| Immunogen Sequence: | SLESLDTSLFAKNPKSKGTK RDTERNKDIAFLEAQIYEYV EILGEQRHLTHENVQRKQAR TGEEREEEEEEQISESESED EENEIIYNPKNLPLGWDGKP IPYWLYKLHGLNINYNCEIC GNYTYRGPKAFQRHFAEWRH AHGMRCLGIPNTAHFANVTQ IEDAVSLWAKLKLQKASERW QPDTEEEYEDSSGNVVNKKT YEDLKRQGLL |
| Specificity: | Recombinant fusion protein containing a sequence corresponding to amino acids 292-501 of human SF3A3 (NP_006793.1). |
| Tissue Specificity | Ubiquitous. |
| Function | Component of the 17S U2 SnRNP complex of the spliceosome, a large ribonucleoprotein complex that removes introns from transcribed pre-mRNAs. The 17S U2 SnRNP complex (1) directly participates in early spliceosome assembly and (2) mediates recognition of the intron branch site during pre-mRNA splicing by promoting the selection of the pre-mRNA branch-site adenosine, the nucleophile for the first step of splicing. Within the 17S U2 SnRNP complex, SF3A3 is part of the SF3A subcomplex that contributes to the assembly of the 17S U2 snRNP, and the subsequent assembly of the pre-spliceosome 'E' complex and the pre-catalytic spliceosome 'A' complex. Involved in pre-mRNA splicing as a component of pre-catalytic spliceosome 'B' complexes. |
| Protein Name | Splicing Factor 3a Subunit 3Sf3a60Spliceosome-Associated Protein 61Sap 61 |
| Database Links | Reactome: R-HSA-72163 |
| Cellular Localisation | Nucleus SpeckleNucleus |
| Alternative Antibody Names | Anti-Splicing Factor 3a Subunit 3 antibodyAnti-Sf3a60 antibodyAnti-Spliceosome-Associated Protein 61 antibodyAnti-Sap 61 antibodyAnti-SF3A3 antibodyAnti-SAP61 antibody |
Information sourced from Uniprot.org
