| Host: |
Rabbit |
| Applications: |
IHC |
| Reactivity: |
Human |
| Note: |
STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description: |
Rabbit polyclonal antibody anti-SETMAR (20-300) is suitable for use in Immunohistochemistry research applications. |
| Clonality: |
Polyclonal |
| Conjugation: |
Unconjugated |
| Isotype: |
IgG |
| Formulation: |
PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3. |
| Purification: |
Affinity purification |
| Dilution Range: |
IHC-P 1:50-1:200 |
| Storage Instruction: |
Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: |
SETMAR |
| Gene ID: |
6419 |
| Uniprot ID: |
SETMR_HUMAN |
| Immunogen Region: |
20-300 |
| Immunogen: |
Recombinant fusion protein containing a sequence corresponding to amino acids 20-300 of human SETMAR (NP_006506.3). |
| Immunogen Sequence: |
KPEAPTEQLDVACGQENLPV GAWPPGAAPAPFQYTPDHVV GPGADIDPTQITFPGCICVK TPCLPGTCSCLRHGENYDDN SCLRDIGSGGKYAEPVFECN VLCRCSDHCRNRVVQKGLQF HFQVFKTHKKGWGLRTLEFI PKGRFVCEYAGEVLGFSEVQ RRIHLQTKSDSNYIIAIREH VYNGQVMETFVDPTYIGNIG RFLNHSCEPNLLMIPVRIDS MVPKLALFAAKDIVPEEEL |
| Tissue Specificity | Widely expressed, with highest expression in placenta and ovary and lowest expression in skeletal muscle. |
| Post Translational Modifications | Methylated. Methylation regulates activity in DNA decatenation. Phosphorylated at Ser-508 by CHEK1 and dephosphorylated by protein phosphatase 2A/PP2A. Phosphorylation at Ser-508 is enhanced by DNA damage and promotes recruitment to damaged DNA. It stimulates DNA repair and impairs replication fork restart. |
| Function | Protein derived from the fusion of a methylase with the transposase of an Hsmar1 transposon that plays a role in DNA double-strand break repair, stalled replication fork restart and DNA integration. DNA-binding protein, it is indirectly recruited to sites of DNA damage through protein-protein interactions. Has also kept a sequence-specific DNA-binding activity recognizing the 19-mer core of the 5'-terminal inverted repeats (TIRs) of the Hsmar1 element and displays a DNA nicking and end joining activity. In parallel, has a histone methyltransferase activity and methylates 'Lys-4' and 'Lys-36' of histone H3. Specifically mediates dimethylation of H3 'Lys-36' at sites of DNA double-strand break and may recruit proteins required for efficient DSB repair through non-homologous end-joining. Also regulates replication fork processing, promoting replication fork restart and regulating DNA decatenation through stimulation of the topoisomerase activity of TOP2A. |
| Protein Name | Histone-Lysine N-Methyltransferase SetmarSet Domain And Mariner Transposase Fusion ProteinMetnase Includes - Histone-Lysine N-Methyltransferase - Transposon Hsmar1 Transposase |
| Cellular Localisation | NucleusChromosomeRecruited On Damaged Dna At Sites Of Double-Strand Breaks |
| Alternative Antibody Names | Anti-Histone-Lysine N-Methyltransferase Setmar antibodyAnti-Set Domain And Mariner Transposase Fusion Protein antibodyAnti-Metnase Includes - Histone-Lysine N-Methyltransferase - Transposon Hsmar1 Transposase antibodyAnti-SETMAR antibody |
Information sourced from Uniprot.org
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
