Anti-SET/I2 alpha PP2A antibody (N-Term) (STJ70055)
SPECIFICATIONS
ClonalityPolyclonal
HostGoat
ConjugationUnconjugated
IsotypeIgG
General Information
| Short Description | Goat polyclonal antibody anti-SET/I2 alpha PP2A (N-Term) is suitable for use in ELISA, Western Blot and Immunohistochemistry research applications. |
| Applications | Pep-ELISA/WB/IHC |
| Host | Goat |
| Reactivity | Human/Cow |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Concentration | 0.5 mg/mL |
| Purification | Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. |
| Dilution Range | Peptide ELISA: antibody detection limit dilution 1:128000.WB: Approx 38kDa band observed in lysates of Human Kidney lysates and of cell lines Daudi and Molt4 (calculated MW of 32.1kDa according to NP_003002.2). Recommended concentration: 0.3-1µg |
| Formulation | 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. NA |
| Storage Instruction | Store at-20°C on receipt and minimise freeze-thaw cycles. |
Target Information
| Gene Symbol | SET |
| Gene ID | 6418 |
| Uniprot ID | SET_HUMAN |
| Accession Number | NP_003002.2 |
| Immunogen Region | N-Term |
| Immunogen Sequence | SAPAAKVSKKELNS |
| Specificity | This antibody is expected to recognize isoform 2 (NP_003002.2). |
Additional Info
| Post Translational Modifications | Isoform 2 is phosphorylated on Ser-15 and Ser-24. Isoform 2 is acetylated on Lys-11. Some glutamate residues are glycylated by TTLL8. This modification occurs exclusively on glutamate residues and results in a glycine chain on the gamma-carboxyl group. N-terminus of isoform 1 is methylated by METTL11A/NTM1. Mainly trimethylated. Isoform 2: Cleaved after Lys-176 by GZMA. The cleavage inhibits its nucleosome assembly activity and disrupts the inhibition on NME1. |
| Function | Multitasking protein, involved in apoptosis, transcription, nucleosome assembly and histone chaperoning. Isoform 2 anti-apoptotic activity is mediated by inhibition of the GZMA-activated DNase, NME1. In the course of cytotoxic T-lymphocyte (CTL)-induced apoptosis, GZMA cleaves SET, disrupting its binding to NME1 and releasing NME1 inhibition. Isoform 1 and isoform 2 are potent inhibitors of protein phosphatase 2A. Isoform 1 and isoform 2 inhibit EP300/CREBBP and PCAF-mediated acetylation of histones (HAT) and nucleosomes, most probably by masking the accessibility of lysines of histones to the acetylases. The predominant target for inhibition is histone H4. HAT inhibition leads to silencing of HAT-dependent transcription and prevents active demethylation of DNA. Both isoforms stimulate DNA replication of the adenovirus genome complexed with viral core proteins.however, isoform 2 specific activity is higher. |
| Protein Name | Protein SetHla-Dr-Associated Protein IiInhibitor Of Granzyme A-Activated DnaseIgaadPhapiiPhosphatase 2a Inhibitor I2pp2aI-2pp2aTemplate-Activating Factor ITaf-I |
| Database Links | Reactome: R-HSA-2299718Reactome: R-HSA-450520 |
| Cellular Localisation | CytoplasmCytosolEndoplasmic ReticulumNucleusNucleoplasmIn The CytoplasmFound Both In The Cytosol And Associated With The Endoplasmic ReticulumThe Set Complex Is Associated With The Endoplasmic ReticulumFollowing Ctl Attack And Cleavage By GzmaMoves Rapidly To The NucleusWhere It Is Found In The NucleoplasmAvoiding The NucleolusSimilar Translocation To The Nucleus Is Also Observed For Lymphocyte-Activated Killer Cells After The Addition Of Calcium |
| Alternative Antibody Names | Anti-Protein Set antibodyAnti-Hla-Dr-Associated Protein Ii antibodyAnti-Inhibitor Of Granzyme A-Activated Dnase antibodyAnti-Igaad antibodyAnti-Phapii antibodyAnti-Phosphatase 2a Inhibitor I2pp2a antibodyAnti-I-2pp2a antibodyAnti-Template-Activating Factor I antibodyAnti-Taf-I antibodyAnti-SET antibody |
Information sourced from Uniprot.org