Anti-sestrin 1 antibody (100-200) [S5MR] (STJ11103135)
SPECIFICATIONS
ClonalityMonoclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
General Information
| Short Description | Rabbit monoclonal sestrin 1 (100-200) antibody for use in WB, IHC-P and ELISA in human and mouse samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications | WB/IHC-P/ELISA |
| Host | Rabbit |
| Reactivity | Human/Mouse |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Monoclonal |
| Clone ID | S5MR |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Concentration | Lot specific |
| Purification | Affinity purification |
| Dilution Range | WB:1:500-1:2000IHC-P:1:50-1:200ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements. |
| Formulation | PBS with 0.02% Sodium Azide, 0.05% BSA, 50% Glycerol, pH 7.3. |
| Storage Instruction | Store at-20ยฐC for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
Target Information
| Gene Symbol | SESN1 |
| Gene ID | 27244 |
| Uniprot ID | SESN1_HUMAN |
| Immunogen Region | 100-200 |
| Immunogen Sequence | KTQHYLLQMDGPLPLHYRHY IGIMAAARHQCSYLVNLHVN DFLHVGGDPKWLNGLENAPQ KLQNLGELNKVLAHRPWLIT KEHIEGLLKAEEHSWSLAEL V |
| Specificity | A synthetic peptide corresponding to a sequence within amino acids 100-200 of human sestrin 1 (Q9Y6P5). |
Additional Info
| Tissue Specificity | Widely expressed. |
| Function | Functions as an intracellular leucine sensor that negatively regulates the TORC1 signaling pathway through the GATOR complex. In absence of leucine, binds the GATOR subcomplex GATOR2 and prevents TORC1 signaling. Binding of leucine to SESN2 disrupts its interaction with GATOR2 thereby activating the TORC1 signaling pathway. This stress-inducible metabolic regulator may also play a role in protection against oxidative and genotoxic stresses. May positively regulate the transcription by NFE2L2 of genes involved in the response to oxidative stress by facilitating the SQSTM1-mediated autophagic degradation of KEAP1. Moreover, may prevent the accumulation of reactive oxygen species (ROS) through the alkylhydroperoxide reductase activity born by the N-terminal domain of the protein. Was originally reported to contribute to oxidative stress resistance by reducing PRDX1. However, this could not be confirmed. |
| Protein Name | Sestrin-1P53-Regulated Protein Pa26 |
| Database Links | Reactome: R-HSA-5628897Reactome: R-HSA-9639288Reactome: R-HSA-9755511 |
| Cellular Localisation | NucleusCytoplasm |
| Alternative Antibody Names | Anti-Sestrin-1 antibodyAnti-P53-Regulated Protein Pa26 antibodyAnti-SESN1 antibodyAnti-PA26 antibodyAnti-SEST1 antibody |
Information sourced from Uniprot.org