Anti-SERPINA1 antibody (1-50 aa) (STJ97256)
SPECIFICATIONS
ClonalityPolyclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
ImmunogenThe antiserum was produced against synthesized peptide derived from the N-terminal region of human SERPINA1 at the amino acid range 1-50
General Information
| Short Description | Rabbit polyclonal anti-Alpha-1-antitrypsin (1-50 aa) for use in WB, IHC, IF and ELISA in Human, Rat and Mouse samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications | WB/IHC/IF/ELISA |
| Host | Rabbit |
| Reactivity | Human/Rat/Mouse |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Concentration | 1 mg/mL |
| Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Dilution Range | WB 1:500-1:2000IHC-P 1:100-1:300ELISA 1:10000IF 1:50-200 |
| Formulation | Liquid in PBS containing 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide. |
| Storage Instruction | Store at-20ยฐC for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
Target Information
| Gene Symbol | SERPINA1 |
| Gene ID | 5265 |
| Uniprot ID | A1AT_HUMAN |
| Immunogen | The antiserum was produced against synthesized peptide derived from the N-terminal region of human SERPINA1 at the amino acid range 1-50 |
| Immunogen Region | 1-50 aa |
| Specificity | AAT Polyclonal Antibody detects endogenous levels of AAT protein. |
Additional Info
| Post Translational Modifications | N-glycosylated. Differential glycosylation produces a number of isoforms. N-linked glycan at Asn-107 is alternatively di-antennary, tri-antennary or tetra-antennary. The glycan at Asn-70 is di-antennary with trace amounts of tri-antennary. Glycan at Asn-271 is exclusively di-antennary. Structure of glycans at Asn-70 and Asn-271 is Hex5HexNAc4. The structure of the antennae is Neu5Ac(alpha1-6)Gal(beta1-4)GlcNAc attached to the core structure Man(alpha1-6)Man(alpha1-3)Man(beta1-4)GlcNAc(beta1-4)GlcNAc. Some antennae are fucosylated, which forms a Lewis-X determinant. Proteolytic processing may yield the truncated form that ranges from Asp-30 to Lys-418. (Microbial infection) Proteolytically processed by Staphylococcus aureus seryl, cysteinyl, and metallo-proteases. |
| Function | Inhibitor of serine proteases. Its primary target is elastase, but it also has a moderate affinity for plasmin and thrombin. Irreversibly inhibits trypsin, chymotrypsin and plasminogen activator. The aberrant form inhibits insulin-induced NO synthesis in platelets, decreases coagulation time and has proteolytic activity against insulin and plasmin. Short peptide from AAT: Reversible chymotrypsin inhibitor. It also inhibits elastase, but not trypsin. Its major physiological function is the protection of the lower respiratory tract against proteolytic destruction by human leukocyte elastase (HLE). |
| Protein Name | Alpha-1-AntitrypsinAlpha-1 Protease InhibitorAlpha-1-AntiproteinaseSerpin A1 Cleaved Into - Short Peptide From AatSpaat |
| Database Links | Reactome: R-HSA-114608Reactome: R-HSA-204005Reactome: R-HSA-381426Reactome: R-HSA-5694530Reactome: R-HSA-6798695Reactome: R-HSA-8957275 |
| Cellular Localisation | SecretedEndoplasmic ReticulumThe S And Z Allele Are Not Secreted Effectively And Accumulate Intracellularly In The Endoplasmic ReticulumShort Peptide From Aat: SecretedExtracellular SpaceExtracellular Matrix |
| Alternative Antibody Names | Anti-Alpha-1-Antitrypsin antibodyAnti-Alpha-1 Protease Inhibitor antibodyAnti-Alpha-1-Antiproteinase antibodyAnti-Serpin A1 Cleaved Into - Short Peptide From Aat antibodyAnti-Spaat antibodyAnti-SERPINA1 antibodyAnti-AAT antibodyAnti-PI antibodyAnti-PRO0684 antibodyAnti-PRO2209 antibody |
Information sourced from Uniprot.org