• Immunofluorescence analysis of U-2 OS cells using Septin 8 Rabbit polyclonal antibody (STJ117206) at dilution of 1:100. Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.
  • Immunofluorescence analysis of L929 cells using Septin 8 Rabbit polyclonal antibody (STJ117206) at dilution of 1:100. Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.
  • Immunofluorescence analysis of C6 cells using Septin 8 Rabbit polyclonal antibody (STJ117206) at dilution of 1:100. Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.
  • Immunohistochemistry analysis of Septin 8 in paraffin-embedded Human thyroid cancer using Septin 8 Rabbit polyclonal antibody (STJ117206) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Western blot analysis of various lysates using Septin 8 Rabbit polyclonal antibody (STJ117206) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.

Anti-SEPT8 antibody (348-427) (STJ117206)

SKU:
STJ117206

€140.63 - €653.13
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Host: Rabbit
Applications: WB/IHC/IF
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-SEPT8 (348-427) is suitable for use in Western Blot, Immunohistochemistry and Immunofluorescence research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.01% Thimerosal, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:1000
IHC-P 1:50-1:200
IF/ICC 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: SEPTIN8
Gene ID: 23176
Uniprot ID: SEPT8_HUMAN
Immunogen Region: 348-427
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 348-427 of human Septin 8 (NP_001287728.1).
Immunogen Sequence: NKVKETELELKEKERELHEK FEHLKRVHQEEKRKVEEKRR ELEEETNAFNRRKAAVEALQ SQALHATSQQPLRKDKDKKN
Tissue Specificity Widely expressed, including in brain, heart and platelets.most abundant in aorta. Isoform 2 is expressed at low levels in specific brain areas, such as occipital pole, frontal lobe, temporal lobe and putamen. Isoform 1 and 3 are highly expressed in specific brain areas, such as occipital pole, frontal lobe, temporal lobe and putamen. Isoform 2 is highly expressed in prostate, testis and ovary. Isoform 1 and isoform 3 are expressed at low levels in prostate, testis and ovary.
Function Filament-forming cytoskeletal GTPase. May play a role in platelet secretion. Seems to participate in the process of SNARE complex formation in synaptic vesicles. Isoform 4: Stabilizes BACE1 protein levels and promotes the sorting and accumulation of BACE1 to the recycling or endosomal compartments, modulating the beta-amyloidogenic processing of APP.
Protein Name Septin-8
Cellular Localisation Cytoplasm
Cytoskeleton
Synapse
Cell Projection
Axon
Cytoplasmic Vesicle
Secretory Vesicle
Synaptic Vesicle Membrane
Presynapse
Expressed In Axons Of Immature Neurons
Localizes To Synapses In Mature Neurons
In Platelets
Found In Areas Surrounding Alpha-Granules
Alternative Antibody Names Anti-Septin-8 antibody
Anti-SEPTIN8 antibody
Anti-KIAA0202 antibody
Anti-SEPT8 antibody

Information sourced from Uniprot.org

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