• Immunofluorescence analysis of U-2 OS cells using Septin 5 Rabbit polyclonal antibody (STJ114819) at dilution of 1:100. Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.
  • Immunohistochemistry analysis of Septin 5 in paraffin-embedded mouse brain using Septin 5 Rabbit polyclonal antibody (STJ114819) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of Septin 5 in paraffin-embedded rat brain using Septin 5 Rabbit polyclonal antibody (STJ114819) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Western blot analysis of various lysates, using Septin 5 Rabbit polyclonal antibody (STJ114819) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.

Anti-SEPT5 antibody (1-369) (STJ114819)

SKU:
STJ114819

£112.50 - £522.50
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Host: Rabbit
Applications: WB/IHC/IF
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-SEPT5 (1-369) is suitable for use in Western Blot, Immunohistochemistry and Immunofluorescence research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.01% Thimerosal, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:1000-1:5000
IHC-P 1:100-1:200
IF/ICC 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: SEPTIN5
Gene ID: 5413
Uniprot ID: SEPT5_HUMAN
Immunogen Region: 1-369
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-369 of human Septin 5 (NP_002679.2).
Immunogen Sequence: MSTGLRYKSKLATPEDKQDI DKQYVGFATLPNQVHRKSVK KGFDFTLMVAGESGLGKSTL VHSLFLTDLYKDRKLLSAEE RISQTVEILKHTVDIEEKGV KLKLTIVDTPGFGDAVNNTE CWKPITDYVDQQFEQYFRDE SGLNRKNIQDNRVHCCLYFI SPFGHGLRPVDVGFMKALHE KVNIVPLIAKADCLVPSEIR KLKERIREEIDKFGIHVYQF PECDSDEDEDFKQQDRELK
Tissue Specificity Expressed at high levels in the CNS, as well as in heart and platelets (at protein level).
Post Translational Modifications Phosphorylated by DYRK1A. In platelets, phosphorylated in response to thrombin, phorbol-12-myristate-13-acetate and collagen.
Function Filament-forming cytoskeletal GTPase. May play a role in cytokinesis (Potential). May play a role in platelet secretion.
Protein Name Septin-5
Cell Division Control-Related Protein 1
Cdcrel-1
Peanut-Like Protein 1
Cellular Localisation Cytoplasm
Cytoskeleton
In Platelets
Found In Areas Surrounding Alpha-Granules
Alternative Antibody Names Anti-Septin-5 antibody
Anti-Cell Division Control-Related Protein 1 antibody
Anti-Cdcrel-1 antibody
Anti-Peanut-Like Protein 1 antibody
Anti-SEPTIN5 antibody
Anti-PNUTL1 antibody
Anti-SEPT5 antibody

Information sourced from Uniprot.org

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